Sheep Apolipoprotein L1 ELISA Kit | APOL1 elisa kit

Sheep Apolipoprotein L1 ELISA Kit

Cat. Num. AAA737306

• Gene Names: APOL1; APOL; APO-L; FSGS4; APOL-I
• Reactivity: Sheep
• Synonyms: Apolipoprotein L1; Sheep Apolipoprotein L1 ELISA Kit; APOL1 elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Intended Uses: This Apo-L1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep Apo-L1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APOL1 elisa kit

Principle of the assay: Apo-L1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Apo-L1 antibody and an Apo-L1-HRP conjugate. The assay sample and buffer are incubated together with Apo-L1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Apo-L1 concentration since Apo-L1 from samples and Apo-L1-HRP conjugate compete for the anti-Apo-L1 antibody binding site. Since the number of sites is limited, as more sites are occupied by Apo-L1 from the sample, fewer sites are left to bind Apo-L1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Apo-L1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for APOL1 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 211938440
• NCBI GeneID: 8542
• NCBI Accession #: NP_001130012.1
• NCBI GenBank Nucleotide #: NM_001136540.1
• Molecular Weight: 42,158 Da
• NCBI Official Full Name: apolipoprotein L1 isoform a
• NCBI Official Synonym Full Names: apolipoprotein L, 1
• NCBI Official Symbol: APOL1
• NCBI Official Synonym Symbols: APOL; APO-L; FSGS4; APOL-I
• NCBI Protein Information: apolipoprotein L1
• UniProt Protein Name: Apolipoprotein L1
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOL1
• UniProt Synonym Gene Names: APOL; Apo-L; ApoL; ApoL-I
• UniProt Entry Name: APOL1_HUMAN

NCBI Description

This gene encodes a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Several different transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]

Uniprot Description

APOL1: May play a role in lipid exchange and transport throughout the body. May participate in reverse cholesterol transport from peripheral cells to the liver. Defects in APOL1 are the cause of focal segmental glomerulosclerosis type 4 (FSGS4). It is a renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. Belongs to the apolipoprotein L family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid-binding; Channel, chloride; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: extracellular space; extracellular region; intrinsic to membrane

Molecular Function: chloride channel activity; protein binding; lipid binding

Biological Process: cholesterol metabolic process; receptor-mediated endocytosis; killing of cells of another organism; cytolysis; innate immune response; chloride transport; lipoprotein metabolic process; lipid transport

Disease: Focal Segmental Glomerulosclerosis 4, Susceptibility To