Goat Growth Differentiation Factor 3 ELISA Kit | GDF3 elisa kit

Goat Growth Differentiation Factor 3 ELISA Kit

Cat. Num. AAA736161

• Gene Names: GDF3; KFS3; MCOP7; MCOPCB6
• Reactivity: Goat
• Synonyms: Growth Differentiation Factor 3; Goat Growth Differentiation Factor 3 ELISA Kit; GDF3 elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This GDF3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat GDF3. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GDF3 elisa kit

Principle of the Assay: GDF3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GDF3 antibody and an GDF3-HRP conjugate. The assay sample and buffer are incubated together with GDF3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GDF3 concentration since GDF3 from samples and GDF3-HRP conjugate compete for the anti-GDF3 antibody binding site. Since the number of sites is limited, as more sites are occupied by GDF3 from the sample, fewer sites are left to bind GDF3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GDF3 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for GDF3 elisa kit

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 10190670
• NCBI GeneID: 9573
• NCBI Accession #: NP_065685.1
• NCBI GenBank Nucleotide #: NM_020634.1
• Molecular Weight: 41,387 Da
• NCBI Official Full Name: growth/differentiation factor 3
• NCBI Official Synonym Full Names: growth differentiation factor 3
• NCBI Official Symbol: GDF3
• NCBI Official Synonym Symbols: KFS3; MCOP7; MCOPCB6
• NCBI Protein Information: growth/differentiation factor 3; GDF-3
• UniProt Protein Name: Growth/differentiation factor 3
• Protein Family: Growth/differentiation factor
• UniProt Gene Name: GDF3
• UniProt Synonym Gene Names: UNQ222/PRO248; GDF-3
• UniProt Entry Name: GDF3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. [provided by RefSeq, Jul 2008]

Uniprot Description

GDF3: Defects in GDF3 are the cause of Klippel-Feil syndrome type 3 (KFS3); also called Klippel-Feil syndrome autosomal dominant 3. KFS3 is a skeletal disorder characterized by congenital fusion of cervical vertebrae. It is due to a failure in the normal segmentation of vertebrae during the early weeks of fetal development. The clinical triad consists of short neck, low posterior hairline, and limited neck movement. Defects in GDF3 are the cause of microphthalmia isolated with coloboma type 6 (MCOPCB6); also called isolated colobomatous microphthalmia 6. MCOPCB6 is a disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataract and other abnormalities like cataract may also be present. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure). Defects in GDF3 are a cause of microphthalmia isolated type 7 (MCOP7). MCOP7 is a disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataract and other abnormalities like cataract may also be present. Belongs to the TGF-beta family.

Protein type: Secreted; Cytokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12p13.1

Cellular Component: extracellular space; cytoplasm

Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding; protein kinase binding

Biological Process: response to dietary excess; in utero embryonic development; notochord development; somite rostral/caudal axis specification; formation of anatomical boundary; negative regulation of BMP signaling pathway; regulation of apoptosis; eye development; regulation of MAPKKK cascade; negative regulation of epidermal cell differentiation; regulation of cell fate commitment; mesoderm development; negative regulation of myoblast differentiation; skeletal development; cell development; growth; endoderm development

Disease: Microphthalmia, Isolated 7; Klippel-feil Syndrome 3, Autosomal Dominant; Microphthalmia, Isolated, With Coloboma 6