Canine Coagulation factor X III ELISA Kit | FX III elisa kit

Canine Coagulation factor X III ELISA Kit

Cat. Num. AAA735617

• Reactivity: Canine
• Synonyms: Coagulation factor X III; Canine Coagulation factor X III ELISA Kit; FX III elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This FX III ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine FX III. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FX III elisa kit

Principle of the Assay: FX III ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FX III antibody and an FX III-HRP conjugate. The assay sample and buffer are incubated together with FX III-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FX III concentration since FX III from samples and FX III-HRP conjugate compete for the anti-FX III antibody binding site. Since the number of sites is limited, as more sites are occupied by FX III from the sample, fewer sites are left to bind FX III-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FX III concentration in each sample is interpolated from this standard curve.

Product Categories/Family for FX III elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 116008305
• Molecular Weight: 5,223 Da
• NCBI Official Full Name: coagulation factor XIII, partial
• UniProt Protein Name: Coagulation factor XIII
• UniProt Gene Name: F13A1
• UniProt Entry Name: A0A2H0_HUMAN

NCBI Description

This gene encodes the coagulation factor XIII A subunit. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. It also crosslinks alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion. [provided by RefSeq, Jul 2008]

Uniprot Description

F13A1: Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl- epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Defects in F13A1 are the cause of factor XIII subunit A deficiency (FA13AD). FA13AD is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women. Belongs to the transglutaminase superfamily. Transglutaminase family.

Protein type: EC 2.3.2.13; Transferase

Chromosomal Location of Human Ortholog: 6p25.3-p24.3

Cellular Component: extracellular region

Molecular Function: protein-glutamine gamma-glutamyltransferase activity; metal ion binding

Biological Process: platelet activation; platelet degranulation; peptide cross-linking; blood coagulation

Disease: Thrombophilia Due To Thrombin Defect; Myocardial Infarction, Susceptibility To; Factor Xiii, A Subunit, Deficiency Of