Bovine Muscarinic acetylcholine receptor M3 ELISA Kit | M-Achr M3 elisa kit

Bovine Muscarinic acetylcholine receptor M3 ELISA Kit

Cat. Num. AAA735236

• Gene Names: CHRM3; HM3; EGBRS
• Reactivity: Bovine
• Synonyms: Muscarinic acetylcholine receptor M3; Bovine Muscarinic acetylcholine receptor M3 ELISA Kit; M-Achr M3 elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This M-Achr M3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine M-Achr M3. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for M-Achr M3 elisa kit

Principle of the assay: M-Achr M3 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for M-Achr M3. Standards or samples are then added to the microtiter plate wells and M-Achr M3 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of M-Achr M3 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for M-Achr M3 are added to each well to “sandwich” the M-Achr M3 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain M-Achr M3 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The M-Achr M3 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for M-Achr M3 elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 4502819
• NCBI GeneID: 1131
• NCBI Accession #: NP_000731.1
• NCBI GenBank Nucleotide #: NM_000740.2
• Molecular Weight: 66,128 Da
• NCBI Official Full Name: muscarinic acetylcholine receptor M3
• NCBI Official Synonym Full Names: cholinergic receptor, muscarinic 3
• NCBI Official Symbol: CHRM3
• NCBI Official Synonym Symbols: HM3; EGBRS
• NCBI Protein Information: muscarinic acetylcholine receptor M3; m3 muscarinic receptor; acetylcholine receptor, muscarinic 3
• UniProt Protein Name: Muscarinic acetylcholine receptor M3
• Protein Family: Muscarinic acetylcholine receptor
• UniProt Gene Name: CHRM3
• UniProt Entry Name: ACM3_HUMAN

NCBI Description

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue. [provided by RefSeq, Jul 2008]

Uniprot Description

mAChR M3: The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. Defects in CHRM3 are the cause of Eagle-Barrett syndrome (EGBRS). EGBRS is a syndrome characterized by thin abdominal musculature with overlying lax skin, cryptorchism, megacystis with disorganized detrusor muscle, and urinary tract abnormalities. Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM3 sub-subfamily.

Protein type: GPCR, family 1; Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 1q43

Cellular Component: asymmetric synapse; postsynaptic membrane; basolateral plasma membrane; integral to plasma membrane; dendrite; plasma membrane; nerve terminal; cell junction

Molecular Function: protein binding; drug binding; receptor activity; acetylcholine binding; phosphoinositide phospholipase C activity; G-protein coupled acetylcholine receptor activity

Biological Process: cell proliferation; G-protein coupled receptor protein signaling pathway; nervous system development; smooth muscle contraction; acetylcholine receptor signaling, muscarinic pathway; regulation of vascular smooth muscle contraction; energy reserve metabolic process; protein modification process; positive regulation of smooth muscle contraction; signal transduction; regulation of insulin secretion; saliva secretion

Disease: Abdominal Muscles, Absence Of, With Urinary Tract Abnormality And Cryptorchidism