Monkey Pirin ELISA Kit | PIR elisa kit

Monkey Pirin ELISA Kit

Cat. Num. AAA734937

• Reactivity: Monkey
• Synonyms: Pirin; Monkey Pirin ELISA Kit; PIR elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 1.0-25ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This PIR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey PIR. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PIR elisa kit

Principle of the Assay: PIR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PIR antibody and an PIR-HRP conjugate. The assay sample and buffer are incubated together with PIR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PIR concentration since PIR from samples and PIR-HRP conjugate compete for the anti-PIR antibody binding site. Since the number of sites is limited, as more sites are occupied by PIR from the sample, fewer sites are left to bind PIR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PIR concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PIR elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 49456601
• Molecular Weight: 32,113 Da
• NCBI Official Full Name: PIR, partial
• UniProt Protein Name: Pirin
• Protein Family: Pirin
• UniProt Gene Name: PIR
• UniProt Synonym Gene Names: Probable quercetinase
• UniProt Entry Name: PIR_HUMAN

Uniprot Description

PIR: Possible transcriptional coregulator. May contribute to the regulation of cellular processes via its interaction with BCL3. May be required for efficient terminal myeloid maturation of hematopoietic cells. May play a role in the regulation of cell migration. May promote apoptosis when overexpressed. Has quercetin 2,3-dioxygenase activity (in vitro). May interact with NF1/CTF1. Interacts with BCL3. Identified in a complex comprised of PIR, BLC3, NFKB1 and target DNA. Up-regulated in CD34(+) cells upon myelomonocytic differentiation. Down-regulated in many acute myeloid leukemias. Up-regulated in primary bronchial epithelial cells exposed to cigarette smoke extract. Highly expressed in a subset of melanomas. Detected at very low levels in most tissues. Expressed in all tissues, with highest level of expression in heart and liver. Inhibited by kojic acid, sodium diethyldithiocarbamate and 1,10-phenanthroline monohydrochloride. Belongs to the pirin family.

Protein type: EC 1.13.11.24

Chromosomal Location of Human Ortholog: Xp22.2

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; metal ion binding; transcription cofactor activity; quercetin 2,3-dioxygenase activity

Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; monocyte differentiation