Typical Testing Data/Standard Curve (for reference only)
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Porcine Transforming Growth Factor beta ELISA Kit | TGFbeta elisa kit
Porcine Transforming Growth Factor beta ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Principle of the Assay: TGF-beta ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-TGF-beta antibody and an TGF-beta-HRP conjugate. The assay sample and buffer are incubated together with TGF-beta-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TGF-beta concentration since TGF-beta from samples and TGF-beta-HRP conjugate compete for the anti-TGF-beta antibody binding site. Since the number of sites is limited, as more sites are occupied by TGF-beta from the sample, fewer sites are left to bind TGF-beta-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TGF-beta concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an RGD-containing protein that binds to type I, II and IV collagens. The RGD motif is found in many extracellular matrix proteins modulating cell adhesion and serves as a ligand recognition sequence for several integrins. This protein plays a role in cell-collagen interactions and may be involved in endochondrial bone formation in cartilage. The protein is induced by transforming growth factor-beta and acts to inhibit cell adhesion. Mutations in this gene are associated with multiple types of corneal dystrophy. [provided by RefSeq, Jul 2008]
Uniprot Description
betaIG-H3: Binds to type I, II, and IV collagens. This adhesion protein may play an important role in cell-collagen interactions. In cartilage, may be involved in endochondral bone formation. By TGFB1. Highly expressed in the corneal epithelium.
Protein type: Extracellular matrix; Cell adhesion; Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 5q31
Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; extracellular region; plasma membrane; basement membrane; trans-Golgi network
Molecular Function: collagen binding; integrin binding; protein binding; extracellular matrix binding
Biological Process: cell proliferation; extracellular matrix organization and biogenesis; visual perception; response to stimulus; chondrocyte differentiation; negative regulation of cell adhesion; angiogenesis; cell adhesion
Disease: Corneal Dystrophy, Epithelial Basement Membrane; Corneal Dystrophy, Lattice Type Iiia; Corneal Dystrophy, Avellino Type; Corneal Dystrophy Of Bowman Layer, Type Ii; Corneal Dystrophy, Lattice Type I; Corneal Dystrophy Of Bowman Layer, Type I; Corneal Dystrophy, Groenouw Type I
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Product Notes
The Porcine TGFbeta tgfbi (Catalog #AAA734482) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA734482 ELISA Kit recognizes Porcine TGFbeta. It is sometimes possible for the material contained within the vial of "Transforming Growth Factor beta, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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