Mouse Hydroxylysine ELISA Kit | Hyl elisa kit

Mouse Hydroxylysine ELISA Kit

Cat. Num. AAA732460

• Gene Names: HYKK; AGPHD1
• Reactivity: Mouse
• Synonyms: Hydroxylysine; Mouse Hydroxylysine ELISA Kit; Hyl elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve

Related Product Information for Hyl elisa kit

Intended Uses: This HYD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse HYD. This ELISA kit for research use only!

Principle of the Assay||HYD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HYD antibody and an HYD-HRP conjugate. The assay sample and buffer are incubated together with HYD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HYD concentration since HYD from samples and HYD-HRP conjugate compete for the anti-HYD antibody binding site. Since the number of sites is limited, as more sites are occupied by HYD from the sample, fewer sites are left to bind HYD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HYD concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 134288902
• NCBI GeneID: 123688
• NCBI Accession #: NP_001077081.1
• NCBI GenBank Nucleotide #: NM_001083612.1
• Molecular Weight: 24,896 Da
• NCBI Official Full Name: hydroxylysine kinase isoform 2
• NCBI Official Synonym Full Names: hydroxylysine kinase
• NCBI Official Symbol: HYKK
• NCBI Official Synonym Symbols: AGPHD1
• NCBI Protein Information: hydroxylysine kinase; 5-hydroxylysine kinase; 5-hydroxy-L-lysine kinase; aminoglycoside phosphotransferase domain containing 1; aminoglycoside phosphotransferase domain-containing protein 1
• UniProt Protein Name: Hydroxylysine kinase
• Protein Family: Hydroxylysine kinase
• UniProt Gene Name: HYKK
• UniProt Synonym Gene Names: AGPHD1; 5-hydroxy-L-lysine kinase
• UniProt Entry Name: HYKK_HUMAN

Uniprot Description

HYKK: Catalyzes the GTP-dependent phosphorylation of 5- hydroxy-L-lysine. Belongs to the aminoglycoside phosphotransferase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.7.1.81; Kinase, other

Chromosomal Location of Human Ortholog: 15q25.1

Cellular Component: cytoplasm

Molecular Function: hydroxylysine kinase activity

Biological Process: phosphorylation