Typical Testing Data/Standard Curve (for reference only)
Human Platelet Factor 4 Variant 1 ELISA Kit | PF4V1 elisa kit
Human Platelet Factor 4 Variant 1 ELISA Kit
Gene Names
PF4V1; PF4A; CXCL4L1; CXCL4V1; PF4-ALT; SCYB4V1
Reactivity
Human
Synonyms
Platelet Factor 4 Variant 1; Human Platelet Factor 4 Variant 1 ELISA Kit; PF4V1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of PF4V1. No significant cross-reactivity or interference between PF4V1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PF4V1 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Competitive or Sandwich
Sensitivity
1.0pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PF4V1 elisa kit
Intended Uses: This PF4V1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PF4V1. This ELISA kit for research use only!
Principle of the Assay: PF4V1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PF4V1. Standards or samples are then added to the microtiter plate wells and PF4V1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PF4V1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PF4V1 are added to each well to "sandwich" the PF4V1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PF4V1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PF4V1 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: PF4V1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PF4V1. Standards or samples are then added to the microtiter plate wells and PF4V1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PF4V1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PF4V1 are added to each well to "sandwich" the PF4V1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PF4V1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PF4V1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for PF4V1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,553 Da
NCBI Official Full Name
platelet factor 4 variant
NCBI Official Synonym Full Names
platelet factor 4 variant 1
NCBI Official Symbol
PF4V1
NCBI Official Synonym Symbols
PF4A; CXCL4L1; CXCL4V1; PF4-ALT; SCYB4V1
NCBI Protein Information
platelet factor 4 variant; PF4alt; PF4var1; C-X-C motif chemokine 4; platelet factor 4, variant 1 (PF4-like)
UniProt Protein Name
Platelet factor 4 variant
Protein Family
UniProt Gene Name
PF4V1
UniProt Synonym Gene Names
CXCL4V1; SCYB4V1
UniProt Entry Name
PF4V_HUMAN
Uniprot Description
PF4V1: Inhibitor of angiogenesis. Inhibitor of endothelial cell chemotaxis (in vitro). Belongs to the intercrine alpha (chemokine CxC) family.
Protein type: Chemokine; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 4q12-q21
Cellular Component: extracellular space
Molecular Function: heparin binding; chemokine activity; CXCR chemokine receptor binding
Biological Process: G-protein coupled receptor protein signaling pathway; positive regulation of leukocyte chemotaxis; immune response; response to lipopolysaccharide; inflammatory response; regulation of cell proliferation
Research Articles on PF4V1
Similar Products
Product Notes
The Human PF4V1 pf4v1 (Catalog #AAA732337) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA732337 ELISA Kit recognizes Human PF4V1. It is sometimes possible for the material contained within the vial of "Platelet Factor 4 Variant 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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