Human Ras related C3 Botulinum Toxin Substrate 1 ELISA Kit | RAC1 elisa kit

Human Ras related C3 Botulinum Toxin Substrate 1 ELISA Kit

Cat. Num. AAA730447

• Gene Names: RAC1; MIG5; Rac-1; TC-25; p21-Rac1
• Reactivity: Human
• Synonyms: Ras related C3 Botulinum Toxin Substrate 1; Human Ras related C3 Botulinum Toxin Substrate 1 ELISA Kit; RAC1 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 5.0-100ng/mL
• Sensitivity: 1.0ng/mL
• Intended Uses: This Rac1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human Rac1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RAC1 elisa kit

Principle of the assay: Rac1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Rac1 antibody and an Rac1-HRP conjugate. The assay sample and buffer are incubated together with Rac1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Rac1 concentration since Rac1 from samples and Rac1-HRP conjugate compete for the anti-Rac1 antibody binding site. Since the number of sites is limited, as more sites are occupied by Rac1 from the sample, fewer sites are left to bind Rac1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Rac1 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 51702787
• NCBI GeneID: 5879
• Molecular Weight: 23,467 Da
• NCBI Official Full Name: Ras-related C3 botulinum toxin substrate 1
• NCBI Official Synonym Full Names: ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
• NCBI Official Symbol: RAC1
• NCBI Official Synonym Symbols: MIG5; Rac-1; TC-25; p21-Rac1
• NCBI Protein Information: ras-related C3 botulinum toxin substrate 1; ras-like protein TC25; cell migration-inducing gene 5 protein
• UniProt Protein Name: Ras-related C3 botulinum toxin substrate 1
• Protein Family: Ras-related protein
• UniProt Gene Name: RAC1
• UniProt Synonym Gene Names: TC25; MIG5
• UniProt Entry Name: RAC1_HUMAN

NCBI Description

The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

RAC1: a plasma membrane-associated member of the Rho-GTPase family. Plays a key role in cytoskeletal reorganization, membrane trafficking, transcriptional regulation and cell growth and development. GTP binding stimulates its activity. Phosphorylation by Akt may inhibit GTP binding of Rac1, therefore attenuating the downstream signal transduction pathway. Found in a trimeric complex composed of DOCK1 and ELMO1, which plays a central role in phagocytosis of apoptotic cells. Two alternatively spliced isoforms have been described.

Protein type: G protein, monomeric; G protein, monomeric, Rho; G protein; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: extrinsic to plasma membrane; Golgi membrane; focal adhesion; membrane; lamellipodium; cytoplasm; melanosome; plasma membrane; actin filament; trans-Golgi network; cytosol; phagocytic cup

Molecular Function: GTPase activity; protein binding; enzyme binding; GTP binding; GTP-dependent protein binding; thioesterase binding; Rho GDP-dissociation inhibitor binding; protein kinase binding; Rab GTPase binding

Biological Process: nerve growth factor receptor signaling pathway; viral reproduction; metabolic process; positive regulation of apoptosis; cell motility involved in cell locomotion; regulation of cell migration; small GTPase mediated signal transduction; positive regulation of stress fiber formation; cell adhesion; bone resorption; platelet activation; anatomical structure morphogenesis; mast cell chemotaxis; dendrite morphogenesis; positive regulation of Rho protein signal transduction; regulation of defense response to virus by virus; negative regulation of interleukin-23 production; T cell costimulation; cerebral cortex radially oriented cell migration; actin cytoskeleton organization and biogenesis; axon guidance; cell-matrix adhesion; localization within membrane; positive regulation of focal adhesion formation; actin filament polymerization; response to wounding; ephrin receptor signaling pathway; inflammatory response; regulation of hydrogen peroxide metabolic process; lamellipodium biogenesis; embryonic olfactory bulb interneuron precursor migration; intercellular junction assembly and maintenance; Wnt receptor signaling pathway, planar cell polarity pathway; positive regulation of phosphoinositide 3-kinase activity; engulfment of apoptotic cell; G-protein coupled receptor protein signaling pathway; cell proliferation; hyperosmotic response; positive regulation of actin filament polymerization; organization of an anatomical structure; auditory receptor cell morphogenesis; ruffle organization and biogenesis; innate immune response; negative regulation of receptor-mediated endocytosis; positive regulation of protein amino acid phosphorylation; cell motility; vascular endothelial growth factor receptor signaling pathway; blood coagulation; positive regulation of DNA replication