Human Glucokinase ELISA Kit | GCK elisa kit

Human Glucokinase ELISA Kit

Cat. Num. AAA729636

• Gene Names: Gck; GLUKA; RNGK2
• Reactivity: Human
• Synonyms: Glucokinase; Human Glucokinase ELISA Kit; GCK elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive or Sandwich
• Detection Range: 2.5-50ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This GCK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GCK. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GCK elisa kit

Principle of the assay: GCK ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for GCK. Standards or samples are then added to the microtiter plate wells and GCK if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of GCK present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for GCK are added to each well to "sandwich" the GCK immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain GCK and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GCK concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 7110599
• NCBI GeneID: 24385
• NCBI Accession #: NP_036697.1
• NCBI GenBank Nucleotide #: NM_012565.2
• Molecular Weight: 51,919 Da
• NCBI Official Full Name: glucokinase isoform 2
• NCBI Official Synonym Full Names: glucokinase
• NCBI Official Symbol: Gck
• NCBI Official Synonym Symbols: GLUKA; RNGK2
• NCBI Protein Information: glucokinase; HK4; HK IV; hexokinase-4; hexokinase-D; hexokinase type IV; glucokinase variant 3
• UniProt Protein Name: Glucokinase
• Protein Family: Glucokinase
• UniProt Gene Name: Gck
• UniProt Synonym Gene Names: HK IV; HK4
• UniProt Entry Name: HXK4_RAT

NCBI Description

catalyzes the conversion of ATP and D-glucose to ADP and D-glucose 6-phosphate; may mediate glucosensing in glucose-inhibited and glucose-excited neurons [RGD, Feb 2006]

Uniprot Description

Catalyzes the initial step in utilization of glucose by the beta-cell and liver at physiological glucose concentration. Pancreatic glucokinase plays an important role in modulating insulin secretion. Hepatic glucokinase helps to facilitate the uptake and conversion of glucose by acting as an insulin-sensitive determinant of hepatic glucose usage.