Typical Testing Data/Standard Curve (for reference only)
Rat Anti-Albumin Antibody ELISA Kit | AAA elisa kit
Rat Anti-Albumin Antibody ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: Samples diluted 1:101 are incubated in the microplates coated with the specific antigen. The antibodies, if present in the specimen, bind to the antigen. The unbound fraction is washed off in the following step. Afterwards anti-human immunoglobulins conjugated to horseradish peroxidase (conjugate) are incubated and react with the antigen-antibody complex of the samples in the microplates. Unbound conjugate is washed off in the following step. Addition of TMBsubstrate generates an enzymatic colorimetric (blue) reaction, which is stopped by diluted acid (color changes to yellow). The intensity of color formation from the chromogen is a function of the amount of conjugate bound to the antigen-antibody complex and this is proportional to the initial concentration of the respective antibodies in the sample!!Background/Introduction: The polymysositis/scleroderma i. e. PM/Scl antigen was first described by Reichlin and Mathioli in 1976. PM/Scl antibodies can be found in sera from individuals suffering from polymyositis/ scleroderma overlap syndrome (PM/Scl). The two major PM-Scl antigens are proteins of 100kDa and 75kDa, termed PM-Scl 100 and PM-Scl 75, respectively. Most anti-PM/Scl-positive sera are reactive with the 100 kDa protein, while some also recognize the 75 kDa protein. The PM-Scl 75 and PM-Scl 100 antigens are part of a multiprotein particle also termed PM-Scl (formerly PM-1). The cellular function of the complete PM-Scl particle has long been an enigma. Recently, the human PM/Scl complex was shown to be related to the yeast exosome, a complex which functions in very diverse pathways, all dealing with RNA degradation, such as mRNA turnover, rRNA processing and sn(o)RNA biogenesis.
NCBI and Uniprot Product Information
NCBI Description
Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin. [provided by RefSeq, Jul 2008]
Uniprot Description
albumin: Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc. Plasma. Belongs to the ALB/AFP/VDB family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Carrier; Secreted
Chromosomal Location of Human Ortholog: 4q13.3
Cellular Component: extracellular space; protein complex; extracellular region; basement membrane; nucleus
Molecular Function: antioxidant activity; toxin binding; protein binding; enzyme binding; copper ion binding; DNA binding; zinc ion binding; chaperone binding; drug binding; oxygen binding; fatty acid binding; pyridoxal phosphate binding
Biological Process: receptor-mediated endocytosis; platelet activation; sodium-independent organic anion transport; bile acid metabolic process; maintenance of mitochondrion localization; lipoprotein metabolic process; hemolysis by symbiont of host red blood cells; cellular response to starvation; response to organic substance; response to mercury ion; bile acid and bile salt transport; retinal homeostasis; platelet degranulation; transport; negative regulation of programmed cell death; blood coagulation; positive regulation of circadian sleep/wake cycle, non-REM sleep; transmembrane transport; response to nutrient; negative regulation of apoptosis
Disease: Analbuminemia; Hyperthyroxinemia, Familial Dysalbuminemic
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Product Notes
The Rat AAA alb (Catalog #AAA729228) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA729228 ELISA Kit recognizes Rat AAA. It is sometimes possible for the material contained within the vial of "Anti-Albumin Antibody, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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