Human Phospholipid Scramblase 1 ELISA Kit | PLSCR1 elisa kit

Human Phospholipid Scramblase 1 ELISA Kit

Cat. Num. AAA729158

• Gene Names: PLSCR1; MMTRA1B
• Reactivity: Human
• Synonyms: Phospholipid Scramblase 1; Human Phospholipid Scramblase 1 ELISA Kit; PLSCR1 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive or Sandwich
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This PLSCR1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PLSCR1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PLSCR1 elisa kit

Principle of the Assay: PLSCR1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PLSCR1. Standards or samples are then added to the microtiter plate wells and PLSCR1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PLSCR1 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for PLSCR1 are added to each well to "sandwich" the PLSCR1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PLSCR1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PLSCR1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PLSCR1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 10863877
• NCBI GeneID: 5359
• NCBI Accession #: NP_066928.1
• NCBI GenBank Nucleotide #: NM_021105.2
• Molecular Weight: 26,825 Da
• NCBI Official Full Name: phospholipid scramblase 1
• NCBI Official Synonym Full Names: phospholipid scramblase 1
• NCBI Official Symbol: PLSCR1
• NCBI Official Synonym Symbols: MMTRA1B
• NCBI Protein Information: phospholipid scramblase 1; PL scramblase 1; erythrocyte phospholipid scramblase; ca(2+)-dependent phospholipid scramblase 1
• UniProt Protein Name: Phospholipid scramblase 1
• Protein Family: Phospholipid scramblase
• UniProt Gene Name: PLSCR1
• UniProt Synonym Gene Names: PL scramblase 1
• UniProt Entry Name: PLS1_HUMAN

Uniprot Description

PLSCR1: May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system. Belongs to the phospholipid scramblase family.

Protein type: Calcium-binding; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q23

Cellular Component: extracellular matrix; Golgi apparatus; membrane; integral to plasma membrane; plasma membrane; nucleolus; cytosol; nucleus; lipid raft

Molecular Function: phospholipid scramblase activity; protein binding; enzyme binding; DNA binding; calcium ion binding; SH3 domain binding; CD4 receptor binding; epidermal growth factor receptor binding

Biological Process: platelet activation; negative regulation of viral genome replication; apoptosis; positive regulation of innate immune response; acute-phase response; positive regulation of transcription from RNA polymerase II promoter; phospholipid scrambling; defense response to virus; regulation of mast cell activation; phosphatidylserine biosynthetic process