Rabbit Periostin ELISA Kit | POSTN elisa kit

Rabbit Periostin ELISA Kit

Cat. Num. AAA728612

• Gene Names: POSTN; PN; OSF2; OSF-2; PDLPOSTN; periostin
• Reactivity: Rabbit
• Synonyms: Periostin; Rabbit Periostin ELISA Kit; POSTN elisa kit

• Reactivity: Rabbit

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 pg/mL.
• Assay Type: Competitive
• Intended Uses: This Periostin ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit Periostin. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Related Product Information for POSTN elisa kit

Principle of the Assay: Periostin ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Periostin antibody and an Periostin-HRP conjugate. The assay sample and buffer are incubated together with Periostin-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Periostin concentration since Periostin from samples and Periostin-HRP conjugate compete for the anti-Periostin antibody binding site. Since the number of sites is limited, as more sites are occupied by Periostin from the sample, fewer sites are left to bind Periostin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Periostin concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 209862907
• NCBI GeneID: 10631
• NCBI Accession #: NP_006466.2
• NCBI GenBank Nucleotide #: NM_006475.2
• Molecular Weight: 80,357 Da
• NCBI Official Full Name: periostin isoform 1
• NCBI Official Synonym Full Names: periostin, osteoblast specific factor
• NCBI Official Symbol: POSTN
• NCBI Official Synonym Symbols: PN; OSF2; OSF-2; PDLPOSTN; periostin
• NCBI Protein Information: periostin; periodontal ligament-specific periostin; osteoblast specific factor 2 (fasciclin I-like)
• UniProt Protein Name: Periostin
• Protein Family: Periostin
• UniProt Gene Name: POSTN
• UniProt Synonym Gene Names: OSF2; PN; OSF-2
• UniProt Entry Name: POSTN_HUMAN

NCBI Description

This gene encodes a secreted extracellular matrix protein that functions in tissue development and regeneration, including wound healing, and ventricular remodeling following myocardial infarction. The encoded protein binds to integrins to support adhesion and migration of epithelial cells. This protein plays a role in cancer stem cell maintenance and metastasis. Mice lacking this gene exhibit cardiac valve disease, and skeletal and dental defects. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

Uniprot Description

POSTN: Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide; Cell adhesion

Chromosomal Location of Human Ortholog: 13q13.3

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; trans-Golgi network

Molecular Function: heparin binding; protein binding; cell adhesion molecule binding

Biological Process: tissue development; extracellular matrix organization and biogenesis; regulation of Notch signaling pathway; cell adhesion; skeletal development