Rat Permeability glycoprotein ELISA Kit | P-gp elisa kit

Rat Permeability glycoprotein ELISA Kit

Cat. Num. AAA727712

• Gene Names: ABCB5; ABCB5beta; EST422562; ABCB5alpha
• Reactivity: Rat
• Synonyms: Permeability glycoprotein; Rat Permeability glycoprotein ELISA Kit; P-gp elisa kit

• Reactivity: Rat

• Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for P-gp elisa kit

Intended Uses: This P-gp ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse P-gp. This ELISA kit for research use only!

Principle of the Assay: P-gp ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-P-gp antibody and an P-gp-HRP conjugate. The assay sample and buffer are incubated together with P-gp-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the P-gp concentration since P-gp from samples and P-gp-HRP conjugate compete for the anti-P-gp antibody binding site. Since the number of sites is limited, as more sites are occupied by P-gp from the sample, fewer sites are left to bind P-gp-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The P-gp concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 33307712
• NCBI GeneID: 340273
• Molecular Weight: 138,641 Da
• NCBI Official Full Name: P-glycoprotein
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family B (MDR/TAP), member 5
• NCBI Official Symbol: ABCB5
• NCBI Official Synonym Symbols: ABCB5beta; EST422562; ABCB5alpha
• NCBI Protein Information: ATP-binding cassette sub-family B member 5; ABCB5 P-gp; P-glycoprotein ABCB5; ATP-binding cassette protein
• UniProt Protein Name: ATP-binding cassette sub-family B member 5
• UniProt Gene Name: ABCB5
• UniProt Entry Name: ABCB5_HUMAN

NCBI Description

ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules (Chen et al., 2005 [PubMed 15760339]).[supplied by OMIM, Mar 2008]

Uniprot Description

ABCB5: Plasma membrane transporter able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Responsible for the resistance to doxorubicin of a subset of malignant melanomas. Belongs to the ABC transporter superfamily. ABCB family. Multidrug resistance exporter (TC 3.A.1.201) subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter, ABC family; Transporter

Chromosomal Location of Human Ortholog: 7p21.1

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: efflux transmembrane transporter activity; ATPase activity, coupled to transmembrane movement of substances; ATP binding

Biological Process: regulation of membrane potential; metabolic process; compound eye corneal lens development; positive regulation of antigen processing and presentation of peptide antigen via MHC class I; cell differentiation; antigen processing and presentation of endogenous peptide antigen via MHC class Ib via ER pathway, TAP-dependent; transmembrane transport; antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent; antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent