Typical Testing Data/Standard Curve (for reference only)
Mouse Growth Differentiation Factor 7 (GDF7) ELISA Kit | GDF7 elisa kit
Mouse Growth Differentiation Factor 7 (GDF7) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: This GDF7 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse GDF7. This ELISA kit for research use only!
Principle of the Assay: GDF7 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GDF7 antibody and an GDF7-HRP conjugate. The assay sample and buffer are incubated together with GDF7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GDF7 concentration since GDF7 from samples and GDF7-HRP conjugate compete for the anti-GDF7 antibody binding site. Since the number of sites is limited, as more sites are occupied by GDF7 from the sample, fewer sites are left to bind GDF7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GDF7 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the CXC subfamily of chemokines. The encoded protein is a secreted growth factor that signal through the G-protein coupled receptor, CXC receptor 2. This protein plays a role in inflammation and as a chemoattractant for neutrophils. Aberrant expression this protein is associated with the growth and progression of certain tumors. A naturally occurring processed form of this protein has increased chemotactic activity. Alternate splicing results in coding and non-coding variants of this gene. A pseudogene of this gene is found on chromosome 4. [provided by RefSeq, Jan 2012]
Uniprot Description
Function: Has chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity. Ref.6 Ref.11
Subcellular location: Secreted.
Post-translational modification: N-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
Sequence similarities: Belongs to the intercrine alpha (chemokine CxC) family.
Research Articles on GDF7
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Product Notes
The Mouse GDF7 cxcl1 (Catalog #AAA727179) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA727179 ELISA Kit recognizes Mouse GDF7. It is sometimes possible for the material contained within the vial of "Growth Differentiation Factor 7 (GDF7), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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