Typical Testing Data/Standard Curve (for reference only)
Human Dopamine beta Hydroxylase ELISA Kit | DBH elisa kit
Human Dopamine beta Hydroxylase ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: DbetaH ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for DbetaH. Standards or samples are then added to the microtiter plate wells and DbetaH if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of DbetaH present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for DbetaH are added to each well to "sandwich" the DbetaH immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain DbetaH and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DbetaH concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. [provided by RefSeq, Jul 2008]
Uniprot Description
DBH: Conversion of dopamine to noradrenaline. Homotetramer composed of two non-covalently bound disulfide-linked dimers. Activity is enhanced by nerve growth factor (in superior cervical ganglia and adrenal medulla). Trans-synaptic stimulation with reserpine, acetylcholine and glucocorticoids. Belongs to the copper type II ascorbate-dependent monooxygenase family.
Protein type: Motility/polarity/chemotaxis; EC 1.14.17.1; Oxidoreductase; Cell cycle regulation; Membrane protein, integral; Apoptosis; Amino Acid Metabolism - tyrosine
Chromosomal Location of Human Ortholog: 9q34
Cellular Component: membrane; cytoplasm; extracellular region; integral to membrane
Molecular Function: copper ion binding; L-ascorbic acid binding; dopamine beta-monooxygenase activity; catalytic activity
Biological Process: fear response; dopamine catabolic process; maternal behavior; response to amphetamine; cytokine production; response to pain; locomotory behavior; behavioral response to ethanol; leukocyte mediated immunity; glucose homeostasis; memory; regulation of cell proliferation; norepinephrine biosynthetic process; synaptic transmission; catecholamine biosynthetic process; homoiothermy; positive regulation of vasoconstriction; visual learning; blood vessel remodeling; leukocyte migration
Disease: Dopamine Beta-hydroxylase Deficiency, Congenital
Research Articles on DBH
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Product Notes
The Human DBH dbh (Catalog #AAA726897) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA726897 ELISA Kit recognizes Human DBH. It is sometimes possible for the material contained within the vial of "Dopamine beta Hydroxylase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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