Mouse Osteonectin ELISA Kit | ON elisa kit

Mouse Osteonectin ELISA Kit

Cat. Num. AAA726287

• Gene Names: SPARC; ON
• Reactivity: Mouse
• Synonyms: Osteonectin; Mouse Osteonectin ELISA Kit; ON elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 10-250ng/mL
• Sensitivity: 1.0ng/mL
• Intended Uses: This ON ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of mouse ON. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ON elisa kit

Principle of the Assay: ON ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ON antibody and an ON-HRP conjugate. The assay sample and buffer are incubated together with ON-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ON concentration since ON from samples and ON-HRP conjugate compete for the anti-ON antibody binding site. Since the number of sites is limited, as more sites are occupied by ON from the sample, fewer sites are left to bind ON-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ON concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ON elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 338325
• NCBI GeneID: 6678
• Molecular Weight: 34,632 Da
• NCBI Official Full Name: osteonectin
• NCBI Official Synonym Full Names: secreted protein, acidic, cysteine-rich (osteonectin)
• NCBI Official Symbol: SPARC
• NCBI Official Synonym Symbols: ON
• NCBI Protein Information: SPARC; BM-40; osteonectin; cysteine-rich protein; basement-membrane protein 40; secreted protein acidic and rich in cysteine
• UniProt Protein Name: SPARC
• UniProt Gene Name: SPARC
• UniProt Synonym Gene Names: ON; BM-40; ON
• UniProt Entry Name: SPRC_HUMAN

NCBI Description

This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. [provided by RefSeq, Dec 2011]

Uniprot Description

SPARC: Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. Belongs to the SPARC family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q31.3-q32

Cellular Component: extracellular space; platelet alpha granule membrane; cell surface; nuclear matrix; mitochondrion; cytoplasm; plasma membrane; extracellular region; basement membrane

Molecular Function: collagen binding; protein binding; extracellular matrix binding; calcium ion binding

Biological Process: platelet activation; response to peptide hormone stimulus; receptor-mediated endocytosis; response to gravity; ossification; extracellular matrix organization and biogenesis; response to cAMP; heart development; response to glucocorticoid stimulus; response to lipopolysaccharide; signal transduction; response to L-ascorbic acid; negative regulation of angiogenesis; platelet degranulation; response to cadmium ion; response to ethanol; response to cytokine stimulus; negative regulation of endothelial cell proliferation; response to lead ion; regulation of cell morphogenesis; response to calcium ion; blood coagulation; inner ear development; lung development

Disease: Osteogenesis Imperfecta, Type Xvii