Typical Testing Data/Standard Curve (for reference only)
Human Alpha 2, 8 sialyltransferase 8E (ST8SIA5) ELISA Kit | ST8SIA5 elisa kit
Human Alpha 2, 8 sialyltransferase 8E (ST8SIA5) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ST8SIA5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ST8SIA5 antibody and an ST8SIA5-HRP conjugate. The assay sample and buffer are incubated together with ST8SIA5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ST8SIA5 concentration since ST8SIA5 from samples and ST8SIA5-HRP conjugate compete for the anti-ST8SIA5 antibody binding site. Since the number of sites is limited, as more sites are occupied by ST8SIA5 from the sample, fewer sites are left to bind ST8SIA5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ST8SIA5 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a type II membrane protein that may be present in the Golgi apparatus. The encoded protein, which is a member of glycosyltransferase family 29, may be involved in the synthesis of gangliosides GD1c, GT1a, GQ1b, and GT3 from GD1a, GT1b, GM1b, and GD3, respectively. [provided by RefSeq, Jul 2008]
Uniprot Description
ST8SIA5: May be involved in the synthesis of gangliosides GD1c, GT1a, GQ1b and GT3 from GD1a, GT1b, GM1b and GD3 respectively. Belongs to the glycosyltransferase 29 family.
Protein type: Membrane protein, integral; Glycan Metabolism - glycosphingolipid biosynthesis - ganglio series; EC 2.4.99.-; Transferase
Chromosomal Location of Human Ortholog: 18q21.1
Cellular Component: Golgi membrane; integral to Golgi membrane
Molecular Function: alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity; sialyltransferase activity
Biological Process: glycosphingolipid biosynthetic process; cellular protein metabolic process; dolichol-linked oligosaccharide biosynthetic process; carbohydrate metabolic process; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification
Research Articles on ST8SIA5
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Product Notes
The Human ST8SIA5 st8sia5 (Catalog #AAA7253412) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7253412 ELISA Kit recognizes Human ST8SIA5. It is sometimes possible for the material contained within the vial of "Alpha 2, 8 sialyltransferase 8E (ST8SIA5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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