Typical Testing Data/Standard Curve (for reference only)
Chicken Acetoacetyl-CoA synthetase (AACS) ELISA Kit | AACS elisa kit
Chicken Acetoacetyl-CoA synthetase (AACS) ELISA Kit
Gene Names
AACS; ACSF1; SUR-5
Reactivity
Chicken
Synonyms
Acetoacetyl-CoA synthetase (AACS); Chicken Acetoacetyl-CoA synthetase (AACS) ELISA Kit; AACS elisa kit
Reactivity
Chicken
Samples
Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for AACS elisa kit
Intended Uses: This A2R1Abs ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of HumanA2R1Abs. This ELISA kit for research use only!
Principle of the Assay: A2R1Abs ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-A2R1Abs antibody and an A2R1Abs-HRP conjugate. The assay sample and buffer are incubated together with A2R1Abs-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the A2R1Abs concentration since A2R1Abs from samples and A2R1Abs-HRP conjugate compete for the anti-A2R1Abs antibody binding site. Since the number of sites is limited, as more sites are occupied by A2R1Abs from the sample, fewer sites are left to bind A2R1Abs-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The A2R1Abs concentration in each sample is interpolated from this standard curve.
Principle of the Assay: A2R1Abs ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-A2R1Abs antibody and an A2R1Abs-HRP conjugate. The assay sample and buffer are incubated together with A2R1Abs-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the A2R1Abs concentration since A2R1Abs from samples and A2R1Abs-HRP conjugate compete for the anti-A2R1Abs antibody binding site. Since the number of sites is limited, as more sites are occupied by A2R1Abs from the sample, fewer sites are left to bind A2R1Abs-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The A2R1Abs concentration in each sample is interpolated from this standard curve.
Product Categories/Family for AACS elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,061 Da
NCBI Official Full Name
acetoacetyl-CoA synthetase
NCBI Official Synonym Full Names
acetoacetyl-CoA synthetase
NCBI Official Symbol
AACS
NCBI Official Synonym Symbols
ACSF1; SUR-5
NCBI Protein Information
acetoacetyl-CoA synthetase; protein sur-5 homolog; acetoacetate-CoA ligase; acyl-CoA synthetase family member 1; homolog of C. elegans supressor of ras 5 (sur-5)
UniProt Protein Name
Acetoacetyl-CoA synthetase
Protein Family
UniProt Gene Name
AACS
UniProt Synonym Gene Names
ACSF1
UniProt Entry Name
AACS_HUMAN
Uniprot Description
AACS: Activates acetoacetate to acetoacetyl-CoA. May be involved in utilizing ketone body for the fatty acid-synthesis during adipose tissue development. Belongs to the ATP-dependent AMP-binding enzyme family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Carbohydrate Metabolism - butanoate; Ligase; EC 6.2.1.16
Chromosomal Location of Human Ortholog: 12q24.31
Cellular Component: cytosol
Molecular Function: butyrate-CoA ligase activity; ATP binding; acetoacetate-CoA ligase activity
Biological Process: response to drug; response to purine; response to starvation; response to oleate; response to ethanol; white fat cell differentiation; positive regulation of insulin secretion; liver development; fatty acid metabolic process; response to nutrient
Research Articles on AACS
Similar Products
Product Notes
The Chicken AACS aacs (Catalog #AAA7251950) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7251950 ELISA Kit recognizes Chicken AACS. It is sometimes possible for the material contained within the vial of "Acetoacetyl-CoA synthetase (AACS), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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