Porcine Heat shock 70 kDa protein 1B (HSPA1B) ELISA Kit | HSPA1B elisa kit

Porcine Heat shock 70 kDa protein 1B (HSPA1B) ELISA Kit

Cat. Num. AAA7250085

• Gene Names: HSPA1B; HSP70-2; HSP70-1B
• Reactivity: Porcine
• Synonyms: Heat shock 70 kDa protein 1B (HSPA1B); Porcine Heat shock 70 kDa protein 1B (HSPA1B) ELISA Kit; HSPA1B elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This HSP701B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine HSP701B. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HSPA1B elisa kit

Priciple of the assay: HSP701B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HSP701B antibody and an HSP701B-HRP conjugate. The assay sample and buffer are incubated together with HSP701B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HSP701B concentration since HSP701B from samples and HSP701B-HRP conjugate compete for the anti-HSP701B antibody binding site. Since the number of sites is limited, as more sites are occupied by HSP701B from the sample, fewer sites are left to bind HSP701B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSP701B concentration in each sample is interpolated from this standard curve.

Product Categories/Family for HSPA1B elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 167466173
• NCBI GeneID: 3304
• NCBI Accession #: NP_005337.2
• NCBI GenBank Nucleotide #: NM_005346.4
• Molecular Weight: 63,937 Da
• NCBI Official Full Name: heat shock 70 kDa protein 1A/1B
• NCBI Official Synonym Full Names: heat shock 70kDa protein 1B
• NCBI Official Symbol: HSPA1B
• NCBI Official Synonym Symbols: HSP70-2; HSP70-1B
• NCBI Protein Information: heat shock 70 kDa protein 1A/1B; HSP70-1/HSP70-2; heat shock 70kD protein 1B; heat shock 70 kDa protein 1/2
• UniProt Protein Name: Heat shock 70 kDa protein 1A/1B
• Protein Family: Heat shock 70 kDa protein
• UniProt Gene Name: HSPA1A
• UniProt Synonym Gene Names: HSPA1; HSX70; HSPA1B; HSP70-1/HSP70-2; HSP70.1/HSP70.2
• UniProt Entry Name: HSP71_HUMAN

NCBI Description

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

HSP70: a critical chaperone protein that has a high affinity for unfolded polypeptide chains. It binds extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In cooperation with other chaperones, hsp70 stabilizes preexistent proteins against aggregation and mediates the folding of newly translated polypeptides in the cytosol as well as within organelles. Mitochondrial HSP70 is crucial to the import process: mutant forms of HSP70 fail to import precursor proteins. Is anti-apoptotic in sympathetic neurones and mediates this effect primarily by suppressing c-Jun transcriptional signalling. Interacts with tau protein and mediates proper folding of tau. Can promote the degradation of tau protein. Triptolide, a potential therapeutic agent for progression/metastasis of pancreatic cancer, causes pancreatic cancer cell death by induction of apoptosis, an effect mediated by the inhibition of HSP70.

Protein type: Motility/polarity/chemotaxis; Chaperone; Heat shock protein

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: signalosome; focal adhesion; mitochondrion; endoplasmic reticulum; inclusion body; ribonucleoprotein complex; cytosol; nucleoplasm; centriole; perinuclear region of cytoplasm; cytoplasm; nuclear speck; nucleus; ubiquitin ligase complex; vesicle

Molecular Function: viral receptor activity; protein binding; enzyme binding; G-protein-coupled receptor binding; ubiquitin protein ligase binding; heat shock protein binding; ATPase activity; unfolded protein binding; double-stranded RNA binding; protein N-terminus binding; ATPase activity, coupled; ATP binding

Biological Process: negative regulation of cell proliferation; entry of virus into host cell; protein stabilization; positive regulation of erythrocyte differentiation; mRNA catabolic process; protein refolding; gene expression; negative regulation of protein ubiquitination; negative regulation of cell growth; response to unfolded protein; negative regulation of apoptosis