Typical Standard Curve/Testing Data
Human Adapter protein CIKS (TRAF3IP2) ELISA Kit | TRAF3IP2 elisa kit
Human Adapter protein CIKS (TRAF3IP2) ELISA Kit
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Principle of the Assay: TRAF3IP2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TRAF3IP2 antibody and an TRAF3IP2-HRP conjugate. The assay sample and buffer are incubated together with TRAF3IP2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TRAF3IP2 concentration since TRAF3IP2 from samples and TRAF3IP2-HRP conjugate compete for the anti-TRAF3IP2 antibody binding site. Since the number of sites is limited, as more sites are occupied by TRAF3IP2 from the sample, fewer sites are left to bind TRAF3IP2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TRAF3IP2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein involved in regulating responses to cytokines by members of the Rel/NF-kappaB transcription factor family. These factors play a central role in innate immunity in response to pathogens, inflammatory signals and stress. This gene product interacts with TRAF proteins (tumor necrosis factor receptor-associated factors) and either I-kappaB kinase or MAP kinase to activate either NF-kappaB or Jun kinase. Several alternative transcripts encoding different isoforms have been identified. Another transcript, which does not encode a protein and is transcribed in the opposite orientation, has been identified. Overexpression of this transcript has been shown to reduce expression of at least one of the protein encoding transcripts, suggesting it has a regulatory role in the expression of this gene. [provided by RefSeq, Aug 2009]
Uniprot Description
TRAF3IP2: Could be involved in the activation of both NF-kappa-B via a NF-kappa-B inhibitor kinase (IKK)-dependent mechanism and stress-activated protein kinase (SAPK)/JNK. Defects in TRAF3IP2 are the cause of susceptibility to psoriasis type 13 (PSORS13). PSORS13 is a common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Apoptosis
Chromosomal Location of Human Ortholog: 6q21
Molecular Function: protein binding
Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; immunoglobulin secretion; B cell apoptosis; humoral immune response
Disease: Candidiasis, Familial, 8; Psoriasis 13, Susceptibility To
Research Articles on TRAF3IP2
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Product Notes
The Human TRAF3IP2 traf3ip2 (Catalog #AAA7249672) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7249672 ELISA Kit recognizes Human TRAF3IP2. It is sometimes possible for the material contained within the vial of "Adapter protein CIKS (TRAF3IP2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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