Typical Testing Data/Standard Curve (for reference only)
Mouse Apoptosis facilitator Bcl-2-like protein 14 (BCL2L14) ELISA Kit | BCL2L14 elisa kit
Mouse Apoptosis facilitator Bcl-2-like protein 14 (BCL2L14) ELISA Kit
Gene Names
BCL2L14; BCLG
Reactivity
Mouse
Synonyms
Apoptosis facilitator Bcl-2-like protein 14 (BCL2L14); Mouse Apoptosis facilitator Bcl-2-like protein 14 (BCL2L14) ELISA Kit; BCL2L14 elisa kit
Reactivity
Mouse
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for BCL2L14 elisa kit
Intended Uses: This BCL2L14 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse BCL2L14. This ELISA kit for research use only!
Principle of the Assay: BCL2L14 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-BCL2L14 antibody and an BCL2L14-HRP conjugate. The assay sample and buffer are incubated together with BCL2L14-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BCL2L14 concentration since BCL2L14 from samples and BCL2L14-HRP conjugate compete for the anti-BCL2L14 antibody binding site. Since the number of sites is limited, as more sites are occupied by BCL2L14 from the sample, fewer sites are left to bind BCL2L14-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BCL2L14 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: BCL2L14 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-BCL2L14 antibody and an BCL2L14-HRP conjugate. The assay sample and buffer are incubated together with BCL2L14-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BCL2L14 concentration since BCL2L14 from samples and BCL2L14-HRP conjugate compete for the anti-BCL2L14 antibody binding site. Since the number of sites is limited, as more sites are occupied by BCL2L14 from the sample, fewer sites are left to bind BCL2L14-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BCL2L14 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for BCL2L14 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,948 Da
NCBI Official Full Name
apoptosis facilitator Bcl-2-like protein 14 isoform 1
NCBI Official Synonym Full Names
BCL2-like 14 (apoptosis facilitator)
NCBI Official Symbol
BCL2L14
NCBI Official Synonym Symbols
BCLG
NCBI Protein Information
apoptosis facilitator Bcl-2-like protein 14; bcl2-L-14; apoptosis regulator BCL-G
UniProt Protein Name
Apoptosis facilitator Bcl-2-like protein 14
Protein Family
UniProt Gene Name
BCL2L14
UniProt Synonym Gene Names
BCLG; Bcl2-L-14
UniProt Entry Name
B2L14_HUMAN
NCBI Description
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Overexpression of this gene has been shown to induce apoptosis in cells. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported for this gene. [provided by RefSeq, May 2009]
Uniprot Description
Bcl-G: Plays a role in apoptosis. Belongs to the Bcl-2 family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Apoptosis
Chromosomal Location of Human Ortholog: 12p13-p12
Cellular Component: membrane; cytoplasm; intracellular organelle; endomembrane system; cytosol
Molecular Function: protein binding; protein kinase binding
Biological Process: regulation of apoptosis; apoptosis
Research Articles on BCL2L14
Similar Products
Product Notes
The Mouse BCL2L14 bcl2l14 (Catalog #AAA7246329) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7246329 ELISA Kit recognizes Mouse BCL2L14. It is sometimes possible for the material contained within the vial of "Apoptosis facilitator Bcl-2-like protein 14 (BCL2L14), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
