Typical Testing Data/Standard Curve (for reference only)
Canine 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 2 (PFKFB2) ELISA Kit | PFKFB2 elisa kit
Canine 6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 2 (PFKFB2) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: PFKFB2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PFKFB2 antibody and an PFKFB2-HRP conjugate. The assay sample and buffer are incubated together with PFKFB2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PFKFB2 concentration since PFKFB2 from samples and PFKFB2-HRP conjugate compete for the anti-PFKFB2 antibody binding site. Since the number of sites is limited, as more sites are occupied by PFKFB2 from the sample, fewer sites are left to bind PFKFB2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PFKFB2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
PFKFB2: a bifunctional heterodimeric enzyme involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. Regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. Two splice-variant isoforms have been described.
Protein type: Motility/polarity/chemotaxis; Carbohydrate Metabolism - fructose and mannose; Kinase, other; Phosphatase (non-protein); EC 3.1.3.46; EC 2.7.1.105
Chromosomal Location of Human Ortholog: 1q31
Cellular Component: cytosol
Molecular Function: protein binding; fructose-2,6-bisphosphate 2-phosphatase activity; 6-phosphofructo-2-kinase activity; protein kinase binding; ATP binding
Biological Process: glucose catabolic process; glycolysis; dephosphorylation; lactate metabolic process; carbohydrate metabolic process; carbohydrate phosphorylation; response to glucose stimulus; glucose metabolic process; positive regulation of insulin secretion; pathogenesis; fructose 2,6-bisphosphate metabolic process; positive regulation of glucokinase activity; fructose metabolic process
Research Articles on PFKFB2
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Product Notes
The Canine PFKFB2 pfkfb2 (Catalog #AAA7241015) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7241015 ELISA Kit recognizes Canine PFKFB2. It is sometimes possible for the material contained within the vial of "6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 2 (PFKFB2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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