Typical Testing Data/Standard Curve (for reference only)
Bovine Chemokine C-X-C-Motif Ligand 1 ELISA Kit | CXCL1 elisa kit
Bovine Chemokine C-X-C-Motif Ligand 1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CCL-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CCL-1 antibody and an CCL-1-HRP conjugate. The assay sample and buffer are incubated together with CCL-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CCL-1 concentration since CCL-1 from samples and CCL-1-HRP conjugate compete for the anti-CCL-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CCL-1 from the sample, fewer sites are left to bind CCL-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCL-1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This secretory protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils. In mouse, deficiency of this gene is associated with colitis and with defects in immune cell recruitment to the lung. [provided by RefSeq, Apr 2013]
Uniprot Description
CXCL2: Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity. Belongs to the intercrine alpha (chemokine CxC) family.
Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted
Cellular Component: extracellular space; cell; extracellular region; cytosol
Molecular Function: growth factor activity; chemokine activity; CXCR chemokine receptor binding; cytokine activity
Biological Process: neutrophil chemotaxis; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; response to molecule of bacterial origin; positive regulation of superoxide release; positive regulation of potassium ion transport; positive regulation of leukocyte chemotaxis; immune response; response to lipopolysaccharide; inflammatory response; acute inflammatory response; regulation of cell proliferation
Research Articles on CXCL1
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Product Notes
The Bovine CXCL1 cxcl1 (Catalog #AAA7239147) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7239147 ELISA Kit recognizes Bovine CXCL1. It is sometimes possible for the material contained within the vial of "Chemokine C-X-C-Motif Ligand 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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