Typical Standard Curve/Testing Data
Mouse Arachidonate 15-lipoxygenase B (ALOX15B) ELISA Kit | ALOX15B elisa kit
Mouse Arachidonate 15-lipoxygenase B (ALOX15B) ELISA Kit
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Principle of the Assay: ALOX15B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ALOX15B antibody and an ALOX15B-HRP conjugate. The assay sample and buffer are incubated together with ALOX15B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALOX15B concentration since ALOX15B from samples and ALOX15B-HRP conjugate compete for the anti-ALOX15B antibody binding site. Since the number of sites is limited, as more sites are occupied by ALOX15B from the sample, fewer sites are left to bind ALOX15B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALOX15B concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
human homolog is an epidermis-type lipoxygenase and member of the multigene family of mammalian lipoxygenases [RGD, Feb 2006]
Uniprot Description
ALOX15B: Converts arachidonic acid exclusively to 15S- hydroperoxyeicosatetraenoic acid, while linoleic acid is less well metabolized. Belongs to the lipoxygenase family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Lipid Metabolism - arachidonic acid; Motility/polarity/chemotaxis; Cell cycle regulation; EC 1.13.11.33; Apoptosis; Oxidoreductase
Cellular Component: cytoskeleton; extrinsic to membrane; membrane; cytoplasm; plasma membrane; intracellular; nucleus; cytosol
Molecular Function: arachidonate 15-lipoxygenase activity; lipoxygenase activity; iron ion binding; calcium ion binding; lipid binding
Biological Process: negative regulation of cell proliferation; lipoxygenase pathway; linoleic acid metabolic process; arachidonic acid metabolic process; hepoxilin biosynthetic process; negative regulation of growth; positive regulation of keratinocyte differentiation; negative regulation of cell cycle
Research Articles on ALOX15B
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Product Notes
The Mouse ALOX15B alox15b (Catalog #AAA7237252) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7237252 ELISA Kit recognizes Mouse ALOX15B. It is sometimes possible for the material contained within the vial of "Arachidonate 15-lipoxygenase B (ALOX15B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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