Human Cysteine and histidine rich domain containing protein 1 (CHORDC1) ELISA Kit | CHORDC1 elisa kit

Human Cysteine and histidine rich domain containing protein 1 (CHORDC1) ELISA Kit

Cat. Num. AAA7236736

• Gene Names: CHORDC1; CHP1
• Reactivity: Human
• Synonyms: Cysteine and histidine rich domain containing protein 1 (CHORDC1); Human Cysteine and histidine rich domain containing protein 1 (CHORDC1) ELISA Kit; CHORDC1 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 1.0 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CHORDC1 elisa kit

Intended Uses: This CHORDC1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CHORDC1. This ELISA kit for research use only!

Principle of the Assay: CHORDC1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CHORDC1 antibody and an CHORDC1-HRP conjugate. The assay sample and buffer are incubated together with CHORDC1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CHORDC1 concentration since CHORDC1 from samples and CHORDC1-HRP conjugate compete for the anti-CHORDC1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CHORDC1 from the sample, fewer sites are left to bind CHORDC1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CHORDC1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CHORDC1 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 221316566
• NCBI GeneID: 26973
• NCBI Accession #: NP_036256.2
• NCBI GenBank Nucleotide #: NM_012124.2
• Molecular Weight: 35,286 Da
• NCBI Official Full Name: cysteine and histidine-rich domain-containing protein 1 isoform a
• NCBI Official Synonym Full Names: cysteine and histidine-rich domain (CHORD) containing 1
• NCBI Official Symbol: CHORDC1
• NCBI Official Synonym Symbols: CHP1
• NCBI Protein Information: cysteine and histidine-rich domain-containing protein 1; CHP-1; protein morgana; CHORD-containing protein 1; chord domain-containing protein 1; cysteine and histidine-rich domain (CHORD)-containing 1; cysteine and histidine-rich domain (CHORD)-containing, zinc-binding protein 1
• UniProt Protein Name: Cysteine and histidine-rich domain-containing protein 1
• Protein Family: Cysteine and histidine-rich domain-containing protein
• UniProt Gene Name: CHORDC1
• UniProt Synonym Gene Names: CHP1; CHORD-containing protein 1; CHP-1
• UniProt Entry Name: CHRD1_HUMAN

Uniprot Description

CHORDC1: Regulates centrosome duplication, probably by inhibiting the kinase activity of ROCK2. Proposed to act as co-chaperone for HSP90. May play a role in the regulation of NOD1 via a HSP90 chaperone complex. In vitro, has intrinsic chaperone activity. This function may be achieved by inhibiting association of ROCK2 with NPM1. Involved in stress response. Prevents tumorigenesis. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 11q14.3

Molecular Function: protein binding; zinc ion binding; Hsp90 protein binding