Mouse Phosphatidylcholine-sterol acyltransferase (LCAT) ELISA Kit | LCAT elisa kit

Mouse Phosphatidylcholine-sterol acyltransferase (LCAT) ELISA Kit

Cat. Num. AAA7236400

• Reactivity: Mouse
• Synonyms: Phosphatidylcholine-sterol acyltransferase (LCAT); Mouse Phosphatidylcholine-sterol acyltransferase (LCAT) ELISA Kit; LCAT elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 1.0 pg/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LCAT elisa kit

Intended Uses: This PMP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PMP. This ELISA kit for research use only!

Principle of the Assay: PMP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-PMP antibody and an PMP-HRP conjugate. The assay sample and buffer are incubated together with PMP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PMP concentration since PMP from samples and PMP-HRP conjugate compete for the anti-PMP antibody binding site. Since the number of sites is limited, as more sites are occupied by PMP from the sample, fewer sites are left to bind PMP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PMP concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557892
• NCBI GeneID: 3931
• NCBI Accession #: NP_000220.1
• NCBI GenBank Nucleotide #: NM_000229.1
• Molecular Weight: 49,578 Da
• NCBI Official Full Name: phosphatidylcholine-sterol acyltransferase
• NCBI Official Synonym Full Names: lecithin-cholesterol acyltransferase
• NCBI Official Symbol: LCAT
• NCBI Protein Information: phosphatidylcholine-sterol acyltransferase; phospholipid-cholesterol acyltransferase
• UniProt Protein Name: Phosphatidylcholine-sterol acyltransferase
• UniProt Gene Name: LCAT
• UniProt Entry Name: LCAT_HUMAN

NCBI Description

This gene encodes the extracellular cholesterol esterifying enzyme, lecithin-cholesterol acyltransferase. The esterification of cholesterol is required for cholesterol transport. Mutations in this gene have been found to cause fish-eye disease as well as LCAT deficiency. [provided by RefSeq, Jul 2008]

Uniprot Description

LCAT: Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms. Defects in LCAT are the cause of lecithin-cholesterol acyltransferase deficiency (LCATD); also called Norum disease. LCATD is a disorder of lipoprotein metabolism characterized by inadequate esterification of plasmatic cholesterol. Two clinical forms are recognized: familial LCAT deficiency and fish-eye disease. Familial LCAT deficiency is associated with a complete absence of alpha and beta LCAT activities and results in esterification anomalies involving both HDL (alpha-LCAT activity) and LDL (beta-LCAT activity). It causes a typical triad of diffuse corneal opacities, target cell hemolytic anemia, and proteinuria with renal failure. Defects in LCAT are a cause of fish-eye disease (FED); also known as dyslipoproteinemic corneal dystrophy or alpha-LCAT deficiency. FED is due to a partial LCAT deficiency that affects only alpha-LCAT activity. It is characterized by low plasma HDL and corneal opacities due to accumulation of cholesterol deposits in the cornea ('fish-eye'). Belongs to the AB hydrolase superfamily. Lipase family.

Protein type: EC 2.3.1.43; Secreted, signal peptide; Lipid Metabolism - glycerophospholipid; Secreted; Transferase

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: extracellular space; extracellular region

Molecular Function: apolipoprotein A-I binding; protein binding; phosphatidylcholine-sterol O-acyltransferase activity

Biological Process: cholesterol metabolic process; cholesterol homeostasis; cholesterol transport; reverse cholesterol transport; lipoprotein biosynthetic process; phospholipid metabolic process; phosphatidylcholine biosynthetic process; lipoprotein metabolic process

Disease: Lecithin:cholesterol Acyltransferase Deficiency; Fish-eye Disease