Typical Testing Data/Standard Curve (for reference only)
Human Conserved oligomeric Golgi complex subunit 5 (COG5) ELISA Kit | COG5 elisa kit
Human Conserved oligomeric Golgi complex subunit 5 (COG5) ELISA Kit
Gene Names
Cog5; GTC90; C87247; D18362; GOLTC1; 5430405C01Rik
Reactivity
Human
Synonyms
Conserved oligomeric Golgi complex subunit 5 (COG5); Human Conserved oligomeric Golgi complex subunit 5 (COG5) ELISA Kit; COG5 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of COG5. No significant cross-reactivity or interference between COG5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between COG5 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for COG5 elisa kit
Intended Uses: This COG5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of HumanCOG5. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: COG5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-COG5 antibody and an COG5-HRP conjugate. The assay sample and buffer are incubated together with COG5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COG5 concentration since COG5 from samples and COG5-HRP conjugate compete for the anti-COG5 antibody binding site. Since the number of sites is limited, as more sites are occupied by COG5 from the sample, fewer sites are left to bind COG5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COG5 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: COG5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-COG5 antibody and an COG5-HRP conjugate. The assay sample and buffer are incubated together with COG5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COG5 concentration since COG5 from samples and COG5-HRP conjugate compete for the anti-COG5 antibody binding site. Since the number of sites is limited, as more sites are occupied by COG5 from the sample, fewer sites are left to bind COG5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COG5 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for COG5 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
91,391 Da
NCBI Official Full Name
conserved oligomeric Golgi complex subunit 5
NCBI Official Synonym Full Names
component of oligomeric golgi complex 5
NCBI Official Symbol
Cog5
NCBI Official Synonym Symbols
GTC90; C87247; D18362; GOLTC1; 5430405C01Rik
NCBI Protein Information
conserved oligomeric Golgi complex subunit 5; COG complex subunit 5
UniProt Protein Name
Conserved oligomeric Golgi complex subunit 5
Protein Family
UniProt Gene Name
Cog5
UniProt Synonym Gene Names
COG complex subunit 5
UniProt Entry Name
COG5_MOUSE
Uniprot Description
COG5: Required for normal Golgi function. Defects in COG5 are the cause of congenital disorder of glycosylation type 2I (CDG2I). A multisystem disorder caused by a defect in glycoprotein biosynthesis and characterized by under-glycosylated serum glycoproteins. Congenital disorders of glycosylation result in a wide variety of clinical features, such as defects in the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency. The broad spectrum of features reflects the critical role of N-glycoproteins during embryonic development, differentiation, and maintenance of cell functions. Congenital disorder of glycosylation type 2I is characterized by mild neurological impairments. Belongs to the COG5 family. 3 isoforms of the human protein are produced by alternative splicing.
Cellular Component: nucleoplasm; Golgi apparatus; membrane; cytoplasm; Golgi transport complex
Biological Process: intra-Golgi vesicle-mediated transport; protein transport; transport; inter-Golgi cisterna vesicle-mediated transport
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Product Notes
The Human COG5 cog5 (Catalog #AAA7235723) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7235723 ELISA Kit recognizes Human COG5. It is sometimes possible for the material contained within the vial of "Conserved oligomeric Golgi complex subunit 5 (COG5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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