Human Sister chromatid cohesion protein PDS5 homolog B (PDS5B) ELISA Kit | PDS5B elisa kit

Human Sister chromatid cohesion protein PDS5 homolog B (PDS5B) ELISA Kit

Cat. Num. AAA7231316

• Gene Names: PDS5B; AS3; APRIN; CG008
• Reactivity: Human
• Synonyms: Sister chromatid cohesion protein PDS5 homolog B (PDS5B); Human Sister chromatid cohesion protein PDS5 homolog B (PDS5B) ELISA Kit; PDS5B elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PDS5B elisa kit

Intended Uses||This PDS5B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PDS5B. This ELISA kit for research use only!

Principle of the Assay: PDS5B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PDS5B antibody and an PDS5B-HRP conjugate. The assay sample and buffer are incubated together with PDS5B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PDS5B concentration since PDS5B from samples and PDS5B-HRP conjugate compete for the anti-PDS5B antibody binding site. Since the number of sites is limited, as more sites are occupied by PDS5B from the sample, fewer sites are left to bind PDS5B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PDS5B concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PDS5B elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 7657269
• NCBI GeneID: 23047
• NCBI Accession #: NP_055847.1
• NCBI GenBank Nucleotide #: NM_015032.3
• Molecular Weight: 14,281 Da
• NCBI Official Full Name: sister chromatid cohesion protein PDS5 homolog B
• NCBI Official Synonym Full Names: PDS5, regulator of cohesion maintenance, homolog B (S. cerevisiae)
• NCBI Official Symbol: PDS5B
• NCBI Official Synonym Symbols: AS3; APRIN; CG008
• NCBI Protein Information: sister chromatid cohesion protein PDS5 homolog B; androgen-induced shutoff 3; androgen-induced proliferation inhibitor; androgen induced inhibitor of proliferation; androgen-induced prostate proliferative shutoff-associated protein AS3
• UniProt Protein Name: Sister chromatid cohesion protein PDS5 homolog B
• Protein Family: Sister chromatid cohesion protein
• UniProt Gene Name: PDS5B
• UniProt Synonym Gene Names: APRIN; AS3; KIAA0979
• UniProt Entry Name: PDS5B_HUMAN

NCBI Description

This gene encodes a protein that interacts with the conserved protein complex termed cohesion. The cohesion complex holds together sister chromatids and facilitates accurate chromosome segregation during mitosis and meiosis. This protein is also a negative regulator of cell proliferation and may be a tumor-suppressor gene. [provided by RefSeq, May 2010]

Uniprot Description

APRIN: a widely expressed nuclear protein. Is lost in many cancers and may function as a tumor suppressor. Correlates with differentiation in embryonal carcinoma stem cells; its knockdown disrupted Nanog, Oct4, and SOX2 regulation. Plays a role in androgen-induced proliferative arrest in prostate cells. Is upregulated in androgen-sensitive LNCaP prostate cancer cells undergoing androgen-induced proliferative block. Required for maintenance of sister chromatid cohesion during mitosis. May couple sister chromatid cohesion during mitosis to DNA replication. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. Belongs to the PDS5 family. Induced by the synthetic androgen R1881 in prostate carcinoma cells undergoing proliferative arrest. Phosphorylated upon DNA damage. Maximum levels occur 18-20 hours after androgen exposure. Interacts with the cohesin complex. Phosphorylated upon DNA damage, probably by ATM or ATR. Five isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; DNA-binding

Chromosomal Location of Human Ortholog: 13q12.3

Cellular Component: nucleoplasm; chromosome; cytosol; chromatin; nucleus; chromosome, pericentric region

Molecular Function: protein binding; DNA binding; ATP binding

Biological Process: cell proliferation; negative regulation of cell proliferation; cell division; mitotic sister chromatid cohesion; mitotic cell cycle; regulation of cell proliferation