Typical Testing Data/Standard Curve (for reference only)
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Mouse Arachidonate 5-lipoxygenase-activating protein (ALOX5AP) ELISA Kit | ALOX5AP elisa kit
Mouse Arachidonate 5-lipoxygenase-activating protein (ALOX5AP) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Principle of the Assay: ALOX5AP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ALOX5AP antibody and an ALOX5AP-HRP conjugate. The assay sample and buffer are incubated together with ALOX5AP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALOX5AP concentration since ALOX5AP from samples and ALOX5AP-HRP conjugate compete for the anti-ALOX5AP antibody binding site. Since the number of sites is limited, as more sites are occupied by ALOX5AP from the sample, fewer sites are left to bind ALOX5AP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALOX5AP concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein which, with 5-lipoxygenase, is required for leukotriene synthesis. Leukotrienes are arachidonic acid metabolites which have been implicated in various types of inflammatory responses, including asthma, arthritis and psoriasis. This protein localizes to the plasma membrane. Inhibitors of its function impede translocation of 5-lipoxygenase from the cytoplasm to the cell membrane and inhibit 5-lipoxygenase activation. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2011]
Uniprot Description
ALOX5AP: Required for leukotriene biosynthesis by ALOX5 (5- lipoxygenase). Anchors ALOX5 to the membrane. Binds arachidonic acid, and could play an essential role in the transfer of arachidonic acid to ALOX5. Binds to MK-886, a compound that blocks the biosynthesis of leukotrienes. Genetic variations in ALOX5AP may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Genetic variations in ALOX5AP may be associated with susceptibility to myocardial infarction. Involvement in myocardial infarction is however unclear: according to some authors (PubMed:14770184), a 4-SNP haplotype in ALOX5AP confers risk of myocardial infarction, while according to other (PubMed:17304054) ALOX5AP is not implicated in this condition. Belongs to the MAPEG family.
Protein type: Activator; Membrane protein, integral; Endoplasmic reticulum; Membrane protein, multi-pass; Nuclear envelope
Chromosomal Location of Human Ortholog: 13q12
Cellular Component: endoplasmic reticulum membrane; nuclear membrane; membrane; endoplasmic reticulum; integral to membrane; nuclear envelope
Molecular Function: arachidonic acid binding; protein binding; glutathione transferase activity; leukotriene-C4 synthase activity; enzyme activator activity; protein N-terminus binding; glutathione peroxidase activity
Biological Process: positive regulation of catalytic activity; leukotriene biosynthetic process; lipoxygenase pathway; arachidonic acid metabolic process; leukotriene metabolic process
Disease: Stroke, Ischemic
Research Articles on ALOX5AP
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Product Notes
The Mouse ALOX5AP alox5ap (Catalog #AAA7231005) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7231005 ELISA Kit recognizes Mouse ALOX5AP. It is sometimes possible for the material contained within the vial of "Arachidonate 5-lipoxygenase-activating protein (ALOX5AP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
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