Human A disintegrin and metalloproteinase with thrombospondin motifs 15 (ADAMTS15) ELISA Kit | ADAMTS15 elisa kit

Human A disintegrin and metalloproteinase with thrombospondin motifs 15 (ADAMTS15) ELISA Kit

Cat. Num. AAA7229568

• Reactivity: Human
• Synonyms: A disintegrin and metalloproteinase with thrombospondin motifs 15 (ADAMTS15); Human A disintegrin and metalloproteinase with thrombospondin motifs 15 (ADAMTS15) ELISA Kit; ADAMTS15 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 1.0-25ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This ADAMTS15 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ADAMTS15. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADAMTS15 elisa kit

Principle of the assay: ADAMTS15 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADAMTS15 antibody and an ADAMTS15-HRP conjugate. The assay sample and buffer are incubated together with ADAMTS15-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADAMTS15 concentration since ADAMTS15 from samples and ADAMTS15-HRP conjugate compete for the anti-ADAMTS15 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADAMTS15 from the sample, fewer sites are left to bind ADAMTS15-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAMTS15 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ADAMTS15 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 21265058
• NCBI GeneID: 170689
• NCBI Accession #: NP_620686.1
• NCBI GenBank Nucleotide #: NM_139055.2
• Molecular Weight: 103,287 Da
• NCBI Official Full Name: A disintegrin and metalloproteinase with thrombospondin motifs 15 preproprotein
• NCBI Official Synonym Full Names: ADAM metallopeptidase with thrombospondin type 1 motif, 15
• NCBI Official Symbol: ADAMTS15
• NCBI Protein Information: A disintegrin and metalloproteinase with thrombospondin motifs 15; ADAM-TS15; ADAMTS-15; ADAM-TS 15; metalloprotease disintegrin 15 with thrombospondin domains; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 15, preproprotein
• UniProt Protein Name: A disintegrin and metalloproteinase with thrombospondin motifs 15
• Protein Family: A disintegrin and metalloproteinase with thrombospondin motifs
• UniProt Gene Name: ADAMTS15
• UniProt Synonym Gene Names: ADAM-TS 15; ADAM-TS15; ADAMTS-15
• UniProt Entry Name: ATS15_HUMAN

NCBI Description

This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. ADAMTS family members share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The protein encoded by this gene has a high sequence similarity to the proteins encoded by ADAMTS1 and ADAMTS8. [provided by RefSeq, Jul 2008]

Uniprot Description

ADAMTS15:

Protein type: Protease; Secreted, signal peptide; Secreted; EC 3.4.24.-; Motility/polarity/chemotaxis; Extracellular matrix

Chromosomal Location of Human Ortholog: 11q25

Cellular Component: proteinaceous extracellular matrix

Molecular Function: zinc ion binding; metalloendopeptidase activity

Biological Process: proteolysis