Typical Testing Data/Standard Curve (for reference only)
Rat Alcohol dehydrogenase class-3 (ADH5) ELISA Kit | ADH5 elisa kit
Rat Alcohol dehydrogenase class-3 (ADH5) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ADH3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ADH3 antibody and an ADH3-HRP conjugate. The assay sample and buffer are incubated together with ADH3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADH3 concentration since ADH3 from samples and ADH3-HRP conjugate compete for the anti-ADH3 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADH3 from the sample, fewer sites are left to bind ADH3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADH3 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The encoded protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. The human genome contains several non-transcribed pseudogenes related to this gene. [provided by RefSeq, Oct 2008]
Uniprot Description
ADH5: Class-III ADH is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.
Protein type: Oxidoreductase; Amino Acid Metabolism - tyrosine; Mitochondrial; Cofactor and Vitamin Metabolism - retinol; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 1.1.1.1; Lipid Metabolism - fatty acid; EC 1.1.1.284; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Energy Metabolism - methane
Chromosomal Location of Human Ortholog: 4q23
Cellular Component: mitochondrion; nucleus
Molecular Function: protein homodimerization activity; electron carrier activity; zinc ion binding; formaldehyde dehydrogenase activity; alcohol dehydrogenase activity; S-(hydroxymethyl)glutathione dehydrogenase activity; fatty acid binding
Biological Process: respiratory system process; response to nitrosative stress; ethanol catabolic process; formaldehyde catabolic process; peptidyl-cysteine S-nitrosylation; response to redox state; response to lipopolysaccharide; ethanol oxidation; retinoid metabolic process; positive regulation of blood pressure; aging
Research Articles on ADH5
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Product Notes
The Rat ADH5 adh5 (Catalog #AAA7227994) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7227994 ELISA Kit recognizes Rat ADH5. It is sometimes possible for the material contained within the vial of "Alcohol dehydrogenase class-3 (ADH5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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