Human Tissue Inhibitor of Metalloproteinases 1 ELISA Kit | TIMP 1 elisa kit

Human Tissue Inhibitor of Metalloproteinases 1 ELISA Kit

Cat. Num. AAA722603

• Reactivity: Human
• Synonyms: Tissue Inhibitor of Metalloproteinases 1; Human Tissue Inhibitor of Metalloproteinases 1 ELISA Kit; TIMP 1 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive or Sandwich
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Highest Standards: 10ng/mL
• Concentration of Standards: Concentration of Standards
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TIMP 1 elisa kit

For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

INTENDED USE This TIMP-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human TIMP-1. This ELISA kit for research use only 2. PRINCIPLE OF THE ASSAY TIMP-1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TIMP-1 Standards or samples are then added to the microtiter plate wells and TIMP-1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TIMP-1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for TIMP-1 are added to each well to “sandwich” the TIMP-1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TIMP-1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TIMP-1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for TIMP 1 elisa kit

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 546974
• NCBI GeneID: 282092
• Molecular Weight: 23,032 Da
• NCBI Official Full Name: TIMP-1
• NCBI Official Synonym Full Names: TIMP metallopeptidase inhibitor 1<
• NCBI Official Symbol: TIMP1
• NCBI Protein Information: metalloproteinase inhibitor 1; EG-1; TIMP-1; embryogenin-1; tissue inhibitor of metalloproteinases 1; tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor)
• UniProt Protein Name: Metalloproteinase inhibitor 1
• UniProt Gene Name: TIMP1
• UniProt Synonym Gene Names: EG-1; TIMP-1
• UniProt Entry Name: TIMP1_BOVIN

Uniprot Description

Metalloproteinase inhibitor that functions by forming one to one complexes with target metalloproteinases, such as collagenases, and irreversibly inactivates them by binding to their catalytic zinc cofactor. Acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP13 and MMP16. Does not act on MMP14. Also functions as a growth factor that regulates cell differentiation, migration and cell death and activates cellular signaling cascades via CD63 and ITGB1. Plays a role in integrin signaling.