Typical Testing Data/Standard Curve (for reference only)
Human Platelet Activating Factor Acetylhydrolase ELISA Kit | PAF AH elisa kit
Human Platelet Activating Factor Acetylhydrolase ELISA Kit
Gene Names
PLA2G7; PAF-AH; Lp-PLA2
Reactivity
Human
Synonyms
Platelet Activating Factor Acetylhydrolase; Human Platelet Activating Factor Acetylhydrolase ELISA Kit; PAF AH elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of PAFA. No significant cross-reactivity or interference between PAFA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PAFA and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive or Sandwich
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PAF AH elisa kit
Intended Uses: This PAFA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PAFA. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: PAFA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PAFA. Standards or samples are then added to the microtiter plate wells and PAFA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PAFA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PAFA are added to each well to "sandwich" the PAFA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PAFA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PAFA concentration in each sample is interpolated from this standard curve.
Principle of the Assay: PAFA ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PAFA. Standards or samples are then added to the microtiter plate wells and PAFA if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PAFA present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PAFA are added to each well to "sandwich" the PAFA immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PAFA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PAFA concentration in each sample is interpolated from this standard curve.
Product Categories/Family for PAF AH elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
platelet-activating factor acetylhydrolase
NCBI Official Symbol
PLA2G7
NCBI Official Synonym Symbols
PAF-AH; Lp-PLA2
NCBI Protein Information
platelet-activating factor acetylhydrolase; group VII phospholipase A2; lipoprotein-associated phospholipase A2
Research Articles on PAF AH
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Product Notes
The Human PAF AH (Catalog #AAA722279) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA722279 ELISA Kit recognizes Human PAF AH. It is sometimes possible for the material contained within the vial of "Platelet Activating Factor Acetylhydrolase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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