Rat Anti-neutrophil cytoplasmic antibody ELISA Kit | CANCA elisa kit

Rat Anti-neutrophil cytoplasmic antibody ELISA Kit

Cat. Num. AAA722228

• Reactivity: Rat
• Synonyms: Anti-neutrophil cytoplasmic antibody; Rat Anti-neutrophil cytoplasmic antibody ELISA Kit; CANCA elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This Caspase-1/ICE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat Caspase-1/ICE. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CANCA elisa kit

Principle of the Assay: Caspase-1/ICE ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Caspase-1/ICE antibody and an Caspase-1/ICE-HRP conjugate. The assay sample and buffer are incubated together with Caspase-1/ICE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Caspase-1/ICE concentration since Caspase-1/ICE from samples and Caspase-1/ICE-HRP conjugate compete for the anti-Caspase-1/ICE antibody binding site. Since the number of sites is limited, as more sites are occupied by Caspase-1/ICE from the sample, fewer sites are left to bind Caspase-1/ICE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Caspase-1/ICE concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CANCA elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 126352357
• NCBI GeneID: 100033888
• NCBI Accession #: NP_001075311.1
• NCBI GenBank Nucleotide #: NM_001081842.1
• Molecular Weight: 45,331 Da
• NCBI Official Full Name: caspase-1
• NCBI Official Symbol: CASP1
• NCBI Protein Information: caspase-1; ICE; p45; CASP-1; IL-1BC; IL-1 beta-converting enzyme; interleukin-1 beta convertase; interleukin-1 beta-converting enzyme; caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
• UniProt Protein Name: Caspase-1
• UniProt Gene Name: CASP1
• UniProt Synonym Gene Names: IL1BC; CASP-1; IL-1BC; ICE; IL-1 beta-converting enzyme
• UniProt Entry Name: CASP1_HORSE

Uniprot Description

Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis ().