Typical Testing Data/Standard Curve (for reference only)
Rat Retinal dehydrogenase 1 (ALDH1A1) ELISA Kit | ALDH1A1 elisa kit
Rat Retinal dehydrogenase 1 (ALDH1A1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ALDH1A1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ALDH1A1 antibody and an ALDH1A1-HRP conjugate. The assay sample and buffer are incubated together with ALDH1A1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALDH1A1 concentration since ALDH1A1 from samples and ALDH1A1-HRP conjugate compete for the anti-ALDH1A1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ALDH1A1 from the sample, fewer sites are left to bind ALDH1A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALDH1A1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet. [provided by RefSeq, Mar 2011]
Uniprot Description
ALDH1A1: Binds free retinal and cellular retinol-binding protein- bound retinal. Can convert/oxidize retinaldehyde to retinoic acid. Belongs to the aldehyde dehydrogenase family.
Protein type: Apoptosis; GAPs; GAPs, Ras; Cofactor and Vitamin Metabolism - retinol; Oxidoreductase; EC 1.2.1.36
Chromosomal Location of Human Ortholog: 9q21.13
Cellular Component: cytoplasm; cytosol
Molecular Function: aldehyde dehydrogenase (NAD) activity; androgen binding; 3-chloroallyl aldehyde dehydrogenase activity; benzaldehyde dehydrogenase (NAD+) activity; retinal dehydrogenase activity
Biological Process: retinol metabolic process; xenobiotic metabolic process; aldehyde metabolic process; ethanol oxidation
Research Articles on ALDH1A1
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Product Notes
The Rat ALDH1A1 aldh1a1 (Catalog #AAA7221903) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7221903 ELISA Kit recognizes Rat ALDH1A1. It is sometimes possible for the material contained within the vial of "Retinal dehydrogenase 1 (ALDH1A1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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