Typical Testing Data/Standard Curve (for reference only)
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Human C type lectin domain family 17, member A (CLEC17A) ELISA Kit | CLEC17A elisa kit
Human C type lectin domain family 17, member A (CLEC17A) ELISA Kit
Reactivity
Human
Synonyms
C type lectin domain family 17; member A (CLEC17A); Human C type lectin domain family 17; member A (CLEC17A) ELISA Kit; CLEC17A elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of CLEC17A. No significant cross-reactivity or interference between CLEC17A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CLEC17A and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for CLEC17A elisa kit
Intended Uses: This CLEC17A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CLEC17A. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: CLEC17A ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CLEC17A antibody and an CLEC17A-HRP conjugate. The assay sample and buffer are incubated together with CLEC17A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLEC17A concentration since CLEC17A from samples and CLEC17A-HRP conjugate compete for the anti-CLEC17A antibody binding site. Since the number of sites is limited, as more sites are occupied by CLEC17A from the sample, fewer sites are left to bind CLEC17A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLEC17A concentration in each sample is interpolated from this standard curve.
Principle of the Assay: CLEC17A ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CLEC17A antibody and an CLEC17A-HRP conjugate. The assay sample and buffer are incubated together with CLEC17A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLEC17A concentration since CLEC17A from samples and CLEC17A-HRP conjugate compete for the anti-CLEC17A antibody binding site. Since the number of sites is limited, as more sites are occupied by CLEC17A from the sample, fewer sites are left to bind CLEC17A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLEC17A concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CLEC17A elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
34,449 Da
NCBI Official Full Name
C-type lectin domain family 17, member A
NCBI Official Synonym Full Names
C-type lectin domain family 17, member A
NCBI Official Symbol
CLEC17A
NCBI Protein Information
C-type lectin domain family 17, member A; prolectin
UniProt Protein Name
C-type lectin domain family 17, member A
Protein Family
UniProt Gene Name
CLEC17A
UniProt Entry Name
CL17A_HUMAN
Uniprot Description
CLEC17A: Cell surface receptor which may be involved in carbohydrate-mediated communication between cells in the germinal center. Binds glycans with terminal alpha-linked mannose or fucose residues. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 19p13.12
Cellular Component: cell surface; integral to membrane
Molecular Function: mannose binding; metal ion binding; fucose binding
Research Articles on CLEC17A
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Product Notes
The Human CLEC17A clec17a (Catalog #AAA7219481) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7219481 ELISA Kit recognizes Human CLEC17A. It is sometimes possible for the material contained within the vial of "C type lectin domain family 17, member A (CLEC17A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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