Rat Aggrecan ELISA Kit | AGC elisa kit
Rat Aggrecan ELISA Kit
Gene Names
Acan; Agc; Agc1
Reactivity
Rat
Synonyms
Aggrecan; Rat Aggrecan ELISA Kit; AGC elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of AGC. No significant cross-reactivity or interference between AGC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AGC and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Sandwich
Sensitivity
1.0pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for AGC elisa kit
Intended Uses: This AGC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat AGC. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: AGC ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AGC. Standards or samples are then added to the microtiter plate wells and AGC if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AGC present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AGC are added to each well to "sandwich" the AGC immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AGC and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGC concentration in each sample is interpolated from this standard curve.
Principle of the Assay: AGC ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AGC. Standards or samples are then added to the microtiter plate wells and AGC if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AGC present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AGC are added to each well to "sandwich" the AGC immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AGC and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGC concentration in each sample is interpolated from this standard curve.
Product Categories/Family for AGC elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
225,171 Da
NCBI Official Full Name
aggrecan core protein
NCBI Official Synonym Full Names
aggrecan
NCBI Official Symbol
Acan
NCBI Official Synonym Symbols
Agc; Agc1
NCBI Protein Information
aggrecan core protein; CSPCP; aggrecan 1; cartilage-specific proteoglycan core protein; aggrecan, structural proteoglycan of cartilage
UniProt Protein Name
Aggrecan core protein
Protein Family
UniProt Gene Name
Acan
UniProt Synonym Gene Names
Agc; Agc1; CSPCP
UniProt Entry Name
PGCA_RAT
NCBI Description
an extracellular matrix proteoglycan; cleaved by ADAMTS1 [RGD, Feb 2006]
Uniprot Description
This proteoglycan is a major component of extracellular matrix of cartilagenous tissues. A major function of this protein is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region. May play a regulatory role in the matrix assembly of the cartilage.
