Typical Standard Curve/Testing Data
Rat NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit 8 (NDUFA8) ELISA Kit
Rat NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit 8 (NDUFA8) ELISA Kit
Reactivity
Rat
Synonyms
NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit 8 (NDUFA8); Rat NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit 8 (NDUFA8) ELISA Kit; NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit 8 (NDUFA8) elisa kit
Reactivity
Rat
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ug/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Related Product Information for NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit 8 (NDUFA8) elisa kit
Intended Uses: This NDUFA8 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat NDUFA8. This ELISA kit for research use only!
Principle of the Assay: NDUFA8 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-NDUFA8 antibody and an NDUFA8-HRP conjugate. The assay sample and buffer are incubated together with NDUFA8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NDUFA8 concentration since NDUFA8 from samples and NDUFA8-HRP conjugate compete for the anti-NDUFA8 antibody binding site. Since the number of sites is limited, as more sites are occupied by NDUFA8 from the sample, fewer sites are left to bind NDUFA8-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NDUFA8 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: NDUFA8 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-NDUFA8 antibody and an NDUFA8-HRP conjugate. The assay sample and buffer are incubated together with NDUFA8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NDUFA8 concentration since NDUFA8 from samples and NDUFA8-HRP conjugate compete for the anti-NDUFA8 antibody binding site. Since the number of sites is limited, as more sites are occupied by NDUFA8 from the sample, fewer sites are left to bind NDUFA8-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NDUFA8 concentration in each sample is interpolated from this standard curve.
Similar Products
Product Notes
The Rat NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit 8 (NDUFA8) (Catalog #AAA7214579) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7214579 ELISA Kit recognizes Rat NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit 8 (NDUFA8). It is sometimes possible for the material contained within the vial of "NADH dehydrogenase ubiquinone 1 alpha subcomplex subunit 8 (NDUFA8), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
