Typical Testing Data/Standard Curve (for reference only)
Mouse Autophagy-related protein 16-1 (ATG16L1) ELISA Kit | ATG16L1 elisa kit
Mouse Autophagy-related protein 16-1 (ATG16L1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ATG16L1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-ATG16L1 antibody and an ATG16L1-HRP conjugate. The assay sample and buffer are incubated together with ATG16L1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATG16L1 concentration since ATG16L1 from samples and ATG16L1-HRP conjugate compete for the anti-ATG16L1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATG16L1 from the sample, fewer sites are left to bind ATG16L1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATG16L1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2010]
Uniprot Description
ATG16: Plays an essential role in autophagy. Homooligomer. Interacts with ATG5. Part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kDa) or 8 sets of ATG12-ATG5 and ATG16L1 (800 kDa). Belongs to the WD repeat ATG16 family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, peripheral; Autophagy; Adaptor/scaffold
Chromosomal Location of Human Ortholog: 2q37.1
Cellular Component: pre-autophagosomal structure membrane; autophagic vacuole; axoneme
Molecular Function: identical protein binding; protein binding
Biological Process: protein transport; protein homooligomerization; autophagic vacuole formation
Disease: Inflammatory Bowel Disease 10
Research Articles on ATG16L1
Similar Products
Product Notes
The Mouse ATG16L1 atg16l1 (Catalog #AAA7211834) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7211834 ELISA Kit recognizes Mouse ATG16L1. It is sometimes possible for the material contained within the vial of "Autophagy-related protein 16-1 (ATG16L1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
