Typical Testing Data/Standard Curve (for reference only)
Rat Cell division control protein 42 homolog (CDC42) ELISA Kit | CDC42 elisa kit
Rat Cell division control protein 42 homolog (CDC42) ELISA Kit
Gene Names
CDC42; G25K; CDC42Hs
Reactivity
Rat
Synonyms
Cell division control protein 42 homolog (CDC42); Rat Cell division control protein 42 homolog (CDC42) ELISA Kit; CDC42 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of CDC42. No significant cross-reactivity or interference between CDC42 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CDC42 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CDC42 elisa kit
Intended Uses: This CDC42 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CDC42. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: CDC42 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CDC42 antibody and an CDC42-HRP conjugate. The assay sample and buffer are incubated together with CDC42-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CDC42 concentration since CDC42 from samples and CDC42-HRP conjugate compete for the anti-CDC42 antibody binding site. Since the number of sites is limited, as more sites are occupied by CDC42 from the sample, fewer sites are left to bind CDC42-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDC42 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: CDC42 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CDC42 antibody and an CDC42-HRP conjugate. The assay sample and buffer are incubated together with CDC42-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CDC42 concentration since CDC42 from samples and CDC42-HRP conjugate compete for the anti-CDC42 antibody binding site. Since the number of sites is limited, as more sites are occupied by CDC42 from the sample, fewer sites are left to bind CDC42-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDC42 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for CDC42 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,311 Da
NCBI Official Full Name
cell division control protein 42 homolog isoform 1
NCBI Official Synonym Full Names
cell division cycle 42
NCBI Official Symbol
CDC42
NCBI Official Synonym Symbols
G25K; CDC42Hs
NCBI Protein Information
cell division control protein 42 homolog; G25K GTP-binding protein; GTP-binding protein, 25kD; growth-regulating protein; GTP binding protein, 25kDa; small GTP binding protein CDC42; dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)); dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD))
UniProt Protein Name
Cell division control protein 42 homolog
Protein Family
UniProt Gene Name
CDC42
UniProt Entry Name
CDC42_HUMAN
NCBI Description
The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20. [provided by RefSeq, Apr 2013]
Research Articles on CDC42
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Product Notes
The Rat CDC42 cdc42 (Catalog #AAA7211423) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7211423 ELISA Kit recognizes Rat CDC42. It is sometimes possible for the material contained within the vial of "Cell division control protein 42 homolog (CDC42), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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