Typical Testing Data/Standard Curve (for reference only)
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Mouse Signal Recognition Particle Antibody ELISA Kit | SRP elisa kit
Mouse Signal Recognition Particle Antibody ELISA Kit
Reactivity
Mouse
Synonyms
Signal Recognition Particle Antibody; Mouse Signal Recognition Particle Antibody ELISA Kit; SRP elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of SRPAb. No significant cross-reactivity or interference between SRPAb and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SRPAb and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for SRP elisa kit
Intended Uses: This SRPAb ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse SRPAb. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: SRPAb ELISA kit applies the competitive enzyme immunoassay technique utilizing a SRP antigen and an SRPAb-HRP conjugate. The assay sample and buffer are incubated together with SRPAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SRPAb concentration since SRPAb from samples and SRPAb-HRP conjugate compete for the GLS antigen binding site. Since the number of sites is limited, as more sites are occupied by SRPAb from the sample, fewer sites are left to bind SRPAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SRPAb concentration in each sample is interpolated from this standard curve.
Principle of the Assay: SRPAb ELISA kit applies the competitive enzyme immunoassay technique utilizing a SRP antigen and an SRPAb-HRP conjugate. The assay sample and buffer are incubated together with SRPAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SRPAb concentration since SRPAb from samples and SRPAb-HRP conjugate compete for the GLS antigen binding site. Since the number of sites is limited, as more sites are occupied by SRPAb from the sample, fewer sites are left to bind SRPAb-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SRPAb concentration in each sample is interpolated from this standard curve.
Product Categories/Family for SRP elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
50,369 Da
NCBI Official Full Name
signal recognition particle
NCBI Official Synonym Full Names
signal recognition particle 54kDa
NCBI Official Symbol
SRP54
NCBI Protein Information
signal recognition particle 54 kDa protein; signal recognition particle 54kD
UniProt Protein Name
Signal recognition particle 54 kDa protein
Protein Family
UniProt Gene Name
SRP54
UniProt Synonym Gene Names
SRP54
UniProt Entry Name
SRP54_HUMAN
Uniprot Description
SRP54: Binds to the signal sequence of presecretory protein when they emerge from the ribosomes and transfers them to TRAM (translocating chain-associating membrane protein). Belongs to the GTP-binding SRP family. SRP54 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: RNA-binding
Chromosomal Location of Human Ortholog: 14q13.2
Cellular Component: cytoplasm; nucleolus; signal recognition particle, endoplasmic reticulum targeting; nuclear speck; cytosol; nucleus
Molecular Function: GTPase activity; ribonucleoprotein binding; GDP binding; GTP binding; drug binding; endoplasmic reticulum signal peptide binding; 7S RNA binding
Biological Process: response to drug; SRP-dependent cotranslational protein targeting to membrane, translocation; protein targeting to ER; SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translation; SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition; gene expression
Research Articles on SRP
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Product Notes
The Mouse SRP srp54 (Catalog #AAA720983) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA720983 ELISA Kit recognizes Mouse SRP. It is sometimes possible for the material contained within the vial of "Signal Recognition Particle Antibody, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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