Human Cadherin 17 (CDH17) ELISA Kit | CDH17 elisa kit

Human Cadherin 17 (CDH17) ELISA Kit

Cat. Num. AAA7207777

• Gene Names: CDH17; HPT1; CDH16; HPT-1
• Reactivity: Human
• Synonyms: Cadherin 17 (CDH17); Human Cadherin 17 (CDH17) ELISA Kit; CDH17 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CDR2L. No significant cross-reactivity or interference between CDR2L and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CDR2L and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CDH17 elisa kit

Intended Uses: This CDR2L ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CDR2L. This ELISA kit for research use only!

Principle of the Assay: CDR2L ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CDR2L antibody and an CDR2L-HRP conjugate. The assay sample and buffer are incubated together with CDR2L-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CDR2L concentration since CDR2L from samples and CDR2L-HRP conjugate compete for the anti-CDR2L antibody binding site. Since the number of sites is limited, as more sites are occupied by CDR2L from the sample, fewer sites are left to bind CDR2L-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDR2L concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CDH17 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 308153624
• NCBI GeneID: 1015
• Molecular Weight: 92,219 Da
• NCBI Official Full Name: Cadherin-17
• NCBI Official Synonym Full Names: cadherin 17, LI cadherin (liver-intestine)
• NCBI Official Symbol: CDH17
• NCBI Official Synonym Symbols: HPT1; CDH16; HPT-1
• NCBI Protein Information: cadherin-17; LI cadherin; LI-cadherin; cadherin-16; HPT-1 cadherin; liver-intestine cadherin; human peptide transporter 1; intestinal peptide-associated transporter HPT-1; human intestinal peptide-associated transporter HPT-1
• UniProt Protein Name: Cadherin-17
• Protein Family: Cadherin
• UniProt Gene Name: CDH17
• UniProt Synonym Gene Names: LI-cadherin
• UniProt Entry Name: CAD17_HUMAN

NCBI Description

This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. The encoded protein is cadherin-like, consisting of an extracellular region, containing 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. The protein may also play a role in the morphological organization of liver and intestine. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2009]

Uniprot Description

CDH17: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. LI-cadherin may have a role in the morphological organization of liver and intestine. Involved in intestinal peptide transport.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 8q22.1

Cellular Component: basolateral plasma membrane; integral to membrane; plasma membrane

Molecular Function: transporter activity; calcium ion binding; proton-dependent oligopeptide secondary active transmembrane transporter activity

Biological Process: intercellular junction assembly and maintenance; transport; calcium-dependent cell-cell adhesion; oligopeptide transport; cell adhesion; homophilic cell adhesion