Canine POU domain, class 5, transcription factor 1 (POU5F1) ELISA Kit | POU5F1 elisa kit

Canine POU domain, class 5, transcription factor 1 (POU5F1) ELISA Kit

Cat. Num. AAA7207610

• Gene Names: POU5F1; OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4
• Reactivity: Canine
• Synonyms: POU domain; class 5; transcription factor 1 (POU5F1); Canine POU domain; transcription factor 1 (POU5F1) ELISA Kit; POU5F1 elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This POU5F1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine POU5F1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

()

Related Product Information for POU5F1 elisa kit

Principle of the Assay: POU5F1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-POU5F1 antibody and an POU5F1-HRP conjugate. The assay sample and buffer are incubated together with POU5F1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the POU5F1 concentration since POU5F1 from samples and POU5F1-HRP conjugate compete for the anti-POU5F1 antibody binding site. Since the number of sites is limited, as more sites are occupied by POU5F1 from the sample, fewer sites are left to bind POU5F1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The POU5F1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for POU5F1 elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 42560248
• NCBI GeneID: 5460
• NCBI Accession #: NP_002692.2
• NCBI GenBank Nucleotide #: NM_002701.5
• Molecular Weight: 30,085 Da
• NCBI Official Full Name: POU domain, class 5, transcription factor 1 isoform 1
• NCBI Official Synonym Full Names: POU class 5 homeobox 1
• NCBI Official Symbol: POU5F1
• NCBI Official Synonym Symbols: OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4
• NCBI Protein Information: POU domain, class 5, transcription factor 1; octamer-binding protein 3; octamer-binding protein 4; POU domain transcription factor OCT4; octamer-binding transcription factor 3; POU-type homeodomain-containing DNA-binding protein
• UniProt Protein Name: POU domain, class 5, transcription factor 1
• Protein Family: POU domain, class 5, transcription factor
• UniProt Gene Name: POU5F1
• UniProt Synonym Gene Names: OCT3; OCT4; OTF3; Oct-3; Oct-4; OTF-3
• UniProt Entry Name: PO5F1_HUMAN

NCBI Description

This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013]

Uniprot Description

Oct4: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. Interacts with UBE2I and ZSCAN10. Interacts with PKM2. Interacts with WWP2. Transcriptional activity is positively regulated by PKM2. Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. Belongs to the POU transcription factor family. Class- 5 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 6p21.31

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; miRNA binding; DNA binding; sequence-specific DNA binding; ubiquitin protein ligase binding; transcription factor binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; anatomical structure morphogenesis; blastocyst development; regulation of transcription, DNA-dependent; regulation of asymmetric cell division; regulation of gene expression; somatic stem cell maintenance; endodermal cell fate specification; response to wounding; positive regulation of transcription from RNA polymerase II promoter; mRNA transcription from RNA polymerase II promoter