Mouse azurocidin ELISA Kit | AZU elisa kit

Mouse azurocidin ELISA Kit

Cat. Num. AAA720075

• Gene Names: AZU1; AZU; HBP; NAZC; AZAMP; CAP37; HUMAZUR
• Reactivity: Mouse
• Synonyms: azurocidin; Mouse azurocidin ELISA Kit; azurocidin (Mouse); AZU elisa kit

• Reactivity: Mouse

• Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AZU elisa kit

For Samples: Cell culture fluid & body fluid & tissue homogenate Serum or blood plasma

Intended Uses: This AZU ELISA kit is intended for laboratory research use only. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of AZU in the sample, this AZU ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus AZU concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of AZU utilizes a multiclonal anti-AZU antibody and an AZU-HRP conjugate. The assay sample and buffer are incubated together with AZU-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AZU concentration since AZU from samples and AZU-HRP conjugate compete for the anti-AZU antibody binding site. Since the number of sites is limited, as more sites are occupied by AZU from the sample, fewer sites are left to bind AZU-HRP conjugate. Standards of known AZU concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (Optical Density) to the concentration of AZU. The AZU concentration in each sample is interpolated from this standard curve.

Product Categories/Family for AZU elisa kit

Mouse ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 11342670
• NCBI GeneID: 566
• NCBI Accession #: NP_001691.1
• NCBI GenBank Nucleotide #: NM_001700.3
• UniProt Accession #: P20160; P80014; Q52LG4; Q9UCM1; Q9UCT5; Q86SR2
• Molecular Weight: 26,886 Da
• NCBI Official Full Name: azurocidin preproprotein
• NCBI Official Synonym Full Names: azurocidin 1
• NCBI Official Symbol: AZU1
• NCBI Official Synonym Symbols: AZU; HBP; NAZC; AZAMP; CAP37; HUMAZUR
• NCBI Protein Information: azurocidin; neutrophil azurocidin; heparin-binding protein; cationic antimicrobial protein 37; cationic antimicrobial protein CAP37
• UniProt Protein Name: Azurocidin
• Protein Family: Azurocidin
• UniProt Gene Name: AZU1
• UniProt Entry Name: CAP7_HUMAN

NCBI Description

Azurophil granules, specialized lysosomes of the neutrophil, contain at least 10 proteins implicated in the killing of microorganisms. The protein encoded by this gene is an azurophil granule antibiotic protein, with monocyte chemotactic and antibacterial activity. It is also an important multifunctional inflammatory mediator. This encoded protein is a member of the serine protease gene family but it is not a serine proteinase, because the active site serine and histidine residues are replaced. The genes encoding this protein, neutrophil elastase 2, and proteinase 3 are in a cluster located at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq]

Uniprot Description

Function: This is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. Binds heparin. The cytotoxic action is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. It may play a role in mediating recruitment of monocytes in the second wave of inflammation. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Acting alone, it does not have antimicrobial activity against the Gram-negative bacteria A.actinomycetemcomitans ATCC 29532, A.actinomycetemcomitans NCTC 9709, A.actinomycetemcomitans FDC-Y4, H.aphrophilus ATCC 13252, E.corrodens ATCC 23834, C.sputigena ATCC 33123, Capnocytophaga sp ATCC 33124, Capnocytophaga sp ATCC 27872 or E.coli ML-35. Has antibacterial activity against C.sputigena ATCC 33123 when acting synergistically with either elastase or cathepsin G. Ref.10 Ref.14

Subunit structure: Monomer.

Subcellular location: Cytoplasmic granule. Note: Cytoplasmic granules of neutrophils.

Sequence similarities: Belongs to the peptidase S1 family. Elastase subfamily.Contains 1 peptidase S1 domain.