Typical Testing Data/Standard Curve (for reference only)
Human Choline/ethanolamine kinase (CHKB) ELISA Kit | CHKB elisa kit
Human Choline/ethanolamine kinase (CHKB) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CHKB ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CHKB antibody and an CHKB-HRP conjugate. The assay sample and buffer are incubated together with CHKB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CHKB concentration since CHKB from samples and CHKB-HRP conjugate compete for the anti-CHKB antibody binding site. Since the number of sites is limited, as more sites are occupied by CHKB from the sample, fewer sites are left to bind CHKB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CHKB concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Choline kinase (CK) and ethanolamine kinase (EK) catalyze the phosphorylation of choline/ethanolamine to phosphocholine/phosphoethanolamine. This is the first enzyme in the biosynthesis of phosphatidylcholine/phosphatidylethanolamine in all animal cells. The highly purified CKs from mammalian sources and their recombinant gene products have been shown to have EK activity also, indicating that both activities reside on the same protein. The choline kinase-like protein encoded by CHKL belongs to the choline/ethanolamine kinase family; however, its exact function is not known. Read-through transcripts are expressed from this locus that include exons from the downstream CPT1B locus. [provided by RefSeq, Jun 2009]
Uniprot Description
CHKB: Has a key role in phospholipid biosynthesis. Catalyzes the first step in phosphatidylethanolamine biosynthesis. Phosphorylates ethanolamine, and can also act on choline (in vitro). Has higher activity with ethanolamine. May not significantly contribute to in vivo phosphatidylcholine biosynthesis. Belongs to the choline/ethanolamine kinase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.7.1.32; Kinase, other; Lipid Metabolism - glycerophospholipid; EC 2.7.1.82
Chromosomal Location of Human Ortholog: 22q13.33
Cellular Component: cytosol
Molecular Function: ethanolamine kinase activity; choline kinase activity; ATP binding
Biological Process: CDP-choline pathway; phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidylethanolamine biosynthetic process; phosphatidylcholine biosynthetic process; phosphorylation
Disease: Muscular Dystrophy, Congenital, Megaconial Type
Research Articles on CHKB
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Product Notes
The Human CHKB chkb (Catalog #AAA7200749) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7200749 ELISA Kit recognizes Human CHKB. It is sometimes possible for the material contained within the vial of "Choline/ethanolamine kinase (CHKB), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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