Rabbit RPT5B Polyclonal Antibody | anti-RPT5B antibody

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RPT5B Polyclonal Antibody

• Gene Names: RPT5B; 26S proteasome AAA-ATPase subunit RPT5B; F7G19.2; F7G19_2
• Reactivity: Arabidopsis thaliana (Mouse-ear cress)
• Applications: ELISA, Western Blot
• Purity: Protein A/G
• Synonyms: RPT5B; Polyclonal Antibody; Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) RPT5B Polyclonal Antibody; RPT5B Antibody; 26S proteasome regulatory subunit 6A homolog B; 26S proteasome AAA-ATPase subunit RPT5b; Proteasome 26S subunit 6A homolog B; Regulatory particle triple-A ATPase subunit 5b; Tat-binding protein 1 homolog B; TBP-1 homolog B; RPT5B TBP1 At1g09100 F7G19.2; anti-RPT5B antibody

• Host: Rabbit
• Reactivity: Arabidopsis thaliana (Mouse-ear cress)
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Protein A/G
• Form/Format: Liquid; Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH7.4

• Applicable Applications for anti-RPT5B antibody: ELISA (EIA), WB (Western Blot) (ensure identification of antigen)
• Immunogen: Recombinant Arabidopsis thaliana RPT5B protein
• Conjugation: Non-conjugate
• Modification: Unmodified
• Deliverables: 1. 200ug recombinant immunogen protein/peptide (positive control).; 2. 1mL preimmune serum (negative control); 3. 10mg rabbit polyclonal antibody purified by Protein A/G.
• Quality Guarantee: 1. Antibody purity >90% confirmed by SDS-PAGE.; 2. Antibody titer > 1: 64,000 confirmed by ELISA; 3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
• Complementary Antibody: Customer can choose 1 FREE antibody from the list below. Please specify the catalog # when order:; * 6*His Monoclonal Antibody #MBS1491670; * DYKDDDDK tag Monoclonal Antibody #MBS1493946; * GAPDH Monoclonal Antibody #MBS1495524; * GFP Monoclonal Antibody #MBS1496050; * Myc tag Monoclonal Antibody #MBS1497694; * GST Monoclonal Antibody #MBS1497871; * Sumo tag Monoclonal Antibody #MBS1498878; * Goat Anti-Rabbit IgG (H+L) Antibody; HRP conjugated #MBS7053510; * HA-Tag Monoclonal Antibody #MBS7135631; * E-Tag Monoclonal Antibody #MBS7135632; * TUBA1A Monoclonal Antibody #MBS7135645; * TUBB Monoclonal Antibody #MBS9018332
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

NCBI and Uniprot Product Information

• NCBI GI #: 15217431
• NCBI GeneID: 837431
• NCBI Accession #: NP_172384.1
• NCBI GenBank Nucleotide #: NM_100781.5
• UniProt Accession #: O04019; Q93V77
• Molecular Weight: 47,038 Da
• NCBI Official Full Name: 26S proteasome AAA-ATPase subunit RPT5B
• NCBI Official Symbol: RPT5B
• NCBI Official Synonym Symbols: 26S proteasome AAA-ATPase subunit RPT5B; F7G19.2; F7G19_2
• NCBI Protein Information: 26S proteasome AAA-ATPase subunit RPT5B
• UniProt Protein Name: 26S proteasome regulatory subunit 6A homolog B
• Protein Family: 26S protease regulatory
• UniProt Gene Name: RPT5B
• UniProt Synonym Gene Names: TBP1; TBP-1 homolog B

NCBI Description

Encodes RPT5b (Regulatory Particle 5b), one of the six AAA-ATPases of the proteasome regulatory particle. Essential for gametophyte development. In Arabidopsis, the RPT5 subunit is encoded by two highly homologous genes, RPT5a and RPT5b. RPT5a and RPT5b show accession-dependent functional redundancy. In Wassilewskija (Ws) accession: mutant alleles of RPT5a displayed 50% pollen lethality, indicating that RPT5a is essential for male gametophyte development. In the Columbia (Col) accession, a rpt5a mutant allele did not display such a phenotype because the RPT5b Col allele complements the rpt5a defect in the male gametophyte, whereas the RPT5b Ws allele does not. Double rpt5a rpt5b mutants in Col background showed a complete male and female gametophyte lethal phenotype.

Uniprot Description

The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.