Rabbit M11P Polyclonal Antibody | anti-M11P antibody

Rabbit anti-Saccharomyces cerevisiae killer virus M1 (ScV-M1)(Saccharomyces cerevisiae virus M1) M11P Polyclonal Antibody

• Reactivity: Saccharomyces cerevisiae killer virus M1 (ScV-M1)(Saccharomyces cerevisiae virus M1)
• Applications: ELISA, Western Blot
• Purity: Protein A/G
• Synonyms: M11P; Polyclonal Antibody; Rabbit anti-Saccharomyces cerevisiae killer virus M1 (ScV-M1)(Saccharomyces cerevisiae virus M1) M11P Polyclonal Antibody; M11P Antibody; M1-1 protoxin; Killer toxin K1; [Cleaved into: M1-1 delta chain; M1-1 alpha chain; M1-1 gamma immunity chain; M1-1 beta chain]; anti-M11P antibody

• Host: Rabbit
• Reactivity: Saccharomyces cerevisiae killer virus M1 (ScV-M1)(Saccharomyces cerevisiae virus M1)
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Protein A/G
• Form/Format: Liquid; Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH7.4

• Applicable Applications for anti-M11P antibody: ELISA (EIA), WB (Western Blot) (ensure identification of antigen)
• Immunogen: Recombinant Saccharomyces cerevisiae killer virus M1 (ScV-M1)(Saccharomyces cerevisiae virus M1) M11P protein
• Conjugation: Non-conjugate
• Modification: Unmodified
• Deliverables: 1. 200ug recombinant immunogen protein/peptide (positive control).; 2. 1mL preimmune serum (negative control); 3. 10mg rabbit polyclonal antibody purified by Protein A/G.
• Quality Guarantee: 1. Antibody purity >90% confirmed by SDS-PAGE.; 2. Antibody titer > 1: 64,000 confirmed by ELISA; 3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
• Complementary Antibody: Customer can choose 1 FREE antibody from the list below. Please specify the catalog # when order:; * 6*His Monoclonal Antibody #MBS1491670; * DYKDDDDK tag Monoclonal Antibody #MBS1493946; * GAPDH Monoclonal Antibody #MBS1495524; * GFP Monoclonal Antibody #MBS1496050; * Myc tag Monoclonal Antibody #MBS1497694; * GST Monoclonal Antibody #MBS1497871; * Sumo tag Monoclonal Antibody #MBS1498878; * Goat Anti-Rabbit IgG (H+L) Antibody; HRP conjugated #MBS7053510; * HA-Tag Monoclonal Antibody #MBS7135631; * E-Tag Monoclonal Antibody #MBS7135632; * TUBA1A Monoclonal Antibody #MBS7135645; * TUBB Monoclonal Antibody #MBS9018332
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

NCBI and Uniprot Product Information

• NCBI GI #: 9629211
• NCBI GeneID: 1494398
• NCBI Accession #: NP_044402.1
• NCBI GenBank Nucleotide #: NC_001782.1
• UniProt Accession #: P01546; NJBYM1
• Molecular Weight: 34,826 Da
• NCBI Official Full Name: preprotoxin
• NCBI Official Symbol: SckvM1gp1
• NCBI Protein Information: preprotoxin
• UniProt Protein Name: M1-1 protoxin
• UniProt Entry Name: M11P_SCVM1

Uniprot Description

Function: Ionophoric toxin secreted by an infected host and lethal to non-infected sensitive strains. Cell killing is achieved in a receptor-mediated process, requiring initial toxin binding to a cell wall (1->6)-beta-D-glucan and, probably, subsequent transfer to a plasma membrane receptor. K1 toxin disrupts the cell by creating a pore across the target cell membrane. Ref.4

Subunit structure: The secreted toxin contains alpha and beta chains that are linked by three disulfide bonds.

Subcellular location: Secreted. Host membrane; Multi-pass membrane protein

Potential. Note: Pore-forming in target cell

Probable.

Miscellaneous: The killer phenotype requires the presence of two different dsRNA viruses: an L-A helper virus and the toxin-coding (M) killer virus. Killer strains of S.cerevisiae producing the toxin K1 kill sensitive cells but are resistant to their own toxin. The gamma and delta chains may play a role, on there own or as part of the protoxin, in the self-protection of the virus-infected killer yeast (toxin immunity).