Rabbit EMRE Polyclonal Antibody | anti-EMRE antibody
Rabbit anti-Escherichia coli (strain K12) EMRE Polyclonal Antibody
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH7.4
2. 1mL preimmune serum (negative control)
3. 10mg rabbit polyclonal antibody purified by Protein A/G.
2. Antibody titer > 1: 64,000 confirmed by ELISA
3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
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NCBI and Uniprot Product Information
NCBI Description
EmrE defunct PDB structures 1S7B and 2F2M, and any conclusions derived from these structures, have been retracted due to a software data inversion error (Ma, 2007; Chang, 2007). [More information is available at EcoGene: EG10629]. EmrE is a multidrug efflux protein with a broad substrate specificity. [More information is available at EcoCyc: EG10629].
Uniprot Description
Function: Multidrug transporter that expels positively charged hydrophobic drugs across the inner membrane of E.coli., thereby conferring resistance to a wide range of toxic compounds. The drug efflux is coupled to an influx of protons. Is involved in the resistance of E.coli cells to methyl viologen, ethidium bromide and acriflavine. Is also able to transport tetraphenylphosphonium (TPP+) and benzalkonium. Ref.6 Ref.8 Ref.13
Subunit structure: Homodimer; parallel. May also form dimer of homodimers. Binds substrate at the dimerization interface. Ref.12 Ref.14 Ref.15 Ref.19 Ref.23
Subcellular location: Cell inner membrane; Multi-pass membrane protein.
Miscellaneous: Encoded by the cryptic lambdoid prophage DLP12.
Sequence similarities: Belongs to the small multidrug resistance (SMR) protein family.
Caution: EM structures show an asymmetric dimer with the monomers in an antiparallel orientation, in contradiction with biochemical data and cross-linking studies, which demonstrated a parallel arrangement. Until now, EmrE with a parallel orientation is the only one to be shown to be fully functional.
Biophysicochemical propertiesKinetic parameters:KM=247 µM for methyl viologen Ref.6Vmax=1572 nmol/min/mg enzyme with methyl viologen as substratepH dependence:Optimum pH is 8-8.5. Transport activity occurs from pH 7.5 to 9.
Research Articles on EMRE
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Product Notes
The EMRE emre (Catalog #AAA7158740) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Escherichia coli (strain K12) EMRE Polyclonal Antibody reacts with E Coli (strain K12) and may cross-react with other species as described in the data sheet. AAA Biotech's EMRE can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), WB (Western Blot) (ensure identification of antigen). Researchers should empirically determine the suitability of the EMRE emre for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EMRE, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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