Rabbit anti-Human Negative elongation factor B Polyclonal Antibody | anti-NELFB antibody

Rabbit anti-human Negative elongation factor B polyclonal Antibody

Cat. Num. AAA715873

• Gene Names: NELFB; COBRA1; NELF-B
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Chromatin Immunoprecipitation, Immunoprecipitation
• Purity: Caprylic Acid Ammonium Sulfate Precipitation Purified
• Synonyms: Negative elongation factor B; Polyclonal Antibody; Rabbit anti-human Negative elongation factor B polyclonal Antibody; Cofactor of BRCA1; anti-NELFB antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Caprylic Acid Ammonium Sulfate Precipitation Purified
• Sequence Length: 628

• Applicable Applications for anti-NELFB antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP)
• Storage Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugate: Non-conjugated
• Immunogen: Recombinant human Negative elongation factor B protein
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-79991 / sc-79992
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(All lanes: Negative elongation factor B antibody at 2ug/mlLane 1: EC109whole cell lysateLane 2: 293T whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 64 kDaObserved band size: 64 kDa)

Western Blot (WB)

(All lanes: Negative elongation factor B antibody at 2ug/mlLane 1: NIH3T3 whole cell lysateLane 2: HL-60 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 64 kDaObserved band size: 64 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human endometrial cancer using MBS715873 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon cancer using MBS715873 at dilution 1:100)

Chromatin Immunoprecipitation (ChIP)

(Chromatin Immunoprecipitation Hela(1.1^106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-COBRA1 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers against the TFF1 promoter.)

Related Product Information for anti-NELFB antibody

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. May be able to induce chromatin unfolding.

NCBI and Uniprot Product Information

• NCBI GI #: 862669351
• NCBI GeneID: 25920
• NCBI Accession #: NP_056271.3
• NCBI GenBank Nucleotide #: NM_015456.4
• UniProt Accession #: Q8WX92; Q96EW5; Q9H9R4; Q9ULN8; Q9Y3W0; A2BFA3
• Molecular Weight: 65,697 Da
• NCBI Official Full Name: negative elongation factor B
• NCBI Official Synonym Full Names: negative elongation factor complex member B
• NCBI Official Symbol: NELFB
• NCBI Official Synonym Symbols: COBRA1; NELF-B
• NCBI Protein Information: negative elongation factor B
• UniProt Protein Name: Negative elongation factor B
• Protein Family: Negative elongation factor
• UniProt Gene Name: NELFB
• UniProt Synonym Gene Names: COBRA1; KIAA1182; NELF-B
• UniProt Entry Name: NELFB_HUMAN

NCBI Description

NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM, Mar 2008]

Uniprot Description

COBRA1: a subunit of the multisubunit transcription elongation factor NELF (negative elongation factor). May be involved in transcriptional pausing of RNA polymerase II (RNAPII). Interacts with the first BRCT repeat of BRCA1.

Protein type: Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: cytoplasm; nucleoplasm

Molecular Function: protein binding

Biological Process: gene expression; positive regulation of viral transcription; RNA elongation from RNA polymerase II promoter; transcription from RNA polymerase II promoter; viral reproduction